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US 2016/0257987 A1Published application
Method for isolating microorganisms from a complex sample
AssigneeMerck Patent GmbH
InventorsMarisa HOHNADEL, Sebastien JOUETTE
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Abstract
The present invention relates to a method for isolating and optionally detecting microorganisms from a sample comprising mammalian cells, comprising the use of a lysis buffer comprising Benzonase® Nuclease. The invention further relates to a lysis buffer comprising Benzonase® Nuclease and its use.
Claims14
Method for isolating microorganisms from a sample comprising mammalian cells, comprising
- a) contacting the sample with a lysis buffer comprising Benzonase® Nuclease,
- b) obtaining the microorganisms by removing the mammalian cells.
Method for isolating and detecting microorganisms from a sample comprising mammalian cells, comprising
- a) contacting the sample with a lysis buffer comprising Benzonase® Nuclease,
- b) obtaining the microorganisms by removing the mammalian cells,
- c) performing a universal lysis on the microorganisms,
- d) performing a specific or universal detection method for detecting the microorganisms.
1 more element
Lysis buffer comprising the following ingredients:
- 0.05 to 0.5% by weight SDS
- 0.5 M to 4 M urea
- 0.5 mM to 10 mM MgCl2
- 2.5 to 175 U Benzonase® Nuclease
- PBS 1×-PBS×⅛.
2 more elements
Dependencies are inferred from the claim text.