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US 2016/0257977 A1Published application
Enhanced Fermentation Process Using a Transglycosidase
AssigneeDanisco US Inc.
InventorsGang Duan, Jayarama K. Shetty, Xin Wang, Hongxian Xu, Xiaoping Zhang, Peng Zhou
Life of this patent
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Abstract
The present invention relates to methods for improving fermentation processes, including increasing product yield, reduced viscosity, and/or reduced foaming.
Claims24
A method of making a fermentation product comprising;
- fermenting a feedstock with a fermenting microorganism in a fermentation broth to make a fermentation product, wherein the fermenting comprises a transglycosidase, and wherein the fermentation product has a higher yield than a control fermentation lacking the transglycosidase.
10-12. (canceled)
A The method of claim 15, wherein
- the fermenting comprises a TrTG Trichoderma transglucosidase, wherein the lysine has a higher yield compared to a control fermentation lacking the Trichoderma transglucosidase, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the Trichoderma transglucosidase.
A The method of claim 15, wherein
- the fermenting comprises an Aspergillus niger transglucosidase, wherein the lysine has a higher yield compared to a control fermentation lacking the Aspergillus niger transglucosidase.
A method of making lysine comprising;
- fermenting a feedstock with Brevibacterium flavum in a fermentation broth to make lysine, wherein the fermenting comprises a transglucosidase, wherein the lysine has a higher yield compared to a control fermentation lacking the transglucosidase;
- or
- fermenting a feedstock with Brevibacterium flavum in a fermentation broth to make lysine, wherein the fermenting comprises a betaglucanase, xylanase and cellulase enzyme complex, wherein the lysine has a higher yield compared to a control fermentation lacking the betaglucanase, xylanase and cellulase enzyme complex.
(canceled)
A method of making glutamic acid comprising;
- fermenting a feedstock with Corynebacterium glutamicum SP 10238 in a fermentation broth to make glutamic acid, wherein the fermenting comprises a TrTG, wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the TrTG, and wherein the fermentation broth has reduced foam compared to a control fermentation lacking the TrTG;
- or
- fermenting a feedstock with Corynebacterium glutamicum SP 10238 in a fermentation broth to make glutamic acid, wherein the fermenting comprises an exoglucanase, a endoglucanase, a β-glucosidase, and a xylanase, wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the exoglucanase, the endoglucanase, the β-glucosidase, and the xylanase, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the exoglucanase, the endoglucanase, the β-glucosidase, and the xylanase.
(canceled)
A method of making lactic acid comprising;
- fermenting a feedstock with Lactobacillus rhamnosus in a fermentation broth to make lactic acid, wherein the fermenting comprises a TrTG, wherein the lactic acid has a higher yield compared to a control fermentation lacking the TrTG, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the TrTG;
- or
- fermenting a feedstock with Lactobacillus rhamnosus in a fermentation broth to make lactic acid, wherein the fermenting comprises a betaglucanase, xylanase and cellulase enzyme complex, wherein the lactic acid has a higher yield compared to a control fermentation lacking the betaglucanase, xylanase and cellulase enzyme complex, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the betaglucanase, xylanase and cellulase enzyme complex.
23-35. (canceled)
Dependencies are inferred from the claim text.