US 2016/0257976 A1 · Biological Synthesis of 6-Aminocaproic Acid and Transgenic Microorganism Therefor

Abstract

The present invention relates to a method for preparing a recombinant microorganism simultaneously comprising genes encoding enzymes used in the biosynthesis pathway of 6-aminocaproic acid, which is a precursor of caprolactam, biosynthesizing 6-aminocaproic acid from the microorganism, and producing the same so as to synthesize caprolactam.

Claims17

Dependent claims

A method for preparing 6-aminocaproic acid comprising:

preparing an expression vector comprising HpaI (4-hydroxy-2-oxoheptane-1,7-dioate aldolase)-HpaH (2-oxohept-3-ene-1,7-dioate dehydratase) gene, nemA (N-ethylmaleimide reductase) gene, and KIVD (alpha-ketoisovalerate decarboxylase) gene;
and at least one of PdAT (beta-alanine-pyruvate transaminase) gene and BcAT (adenosylmethionine-8-amino-7-oxononanoate aminotransferase) gene (step 1);
and
transforming the expression vector of step 1 into a microorganism (step 2).

1 more element

Dependencies are inferred from the claim text.

Facts

Application

14/889,543Filed 2 May 2014

Priority

KR 10-2013-00506766 May 2013

Published

8 Sep 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12P 13/005
Preparation of nitrogen-containing organic compounds: amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
C07D 223/10
Oxygen atoms: attached in position 2
C12N 9/0008
Oxidoreductases: acting on the aldehyde or oxo group of donors
C12N 9/1029
Acyltransferases: transferring groups other than amino-acyl groups

Biological Synthesis of 6-Aminocaproic Acid and Transgenic Microorganism Therefor

Life of this patent

Biological Synthesis of 6-Aminocaproic Acid and Transgenic Microorganism Therefor

Life of this patent

Abstract

Claims17