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AssigneeSynthetic Genomics, Inc.
InventorsNICKY C. CAIAZZA, MAUNG NYAN WIN, JUN URANO
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The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.
A nucleic acid construct comprising a nucleic acid sequence that comprises a promoter, wherein the nucleic acid sequence:
An amplification reaction mixture comprising primers adapted for amplifying a nucleic acid comprising at least 50 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID Nos:1-70, SEQ ID Nos:180-202, complements thereof, and nucleic acids exhibiting at least 80% sequence identity thereto.
A ligation reaction mixture comprising a nucleic acid comprising at least 50 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID Nos:1-70, SEQ ID Nos:180-202, complements thereof, and nucleic acids exhibiting at least 80% sequence identity thereto.
Dependencies are inferred from the claim text.