US 2016/0257959 A1 · NOVEL BIO-SENSOR FOR THE DETECTION OF SMALL MOLECULES

Abstract

This present invention provides methods for the detection of small molecules in samples comprising the steps a) coupling a nanoparticle (NP) or microparticle (MP) to an aptamer specific for the small molecule to be detected to form a NP-aptamer conjugate b) contacting the NP-aptamer conjugate with the sample; and c) detecting a change in the size, surface potential, or mobility of the NP-aptamer conjugate, wherein the change is indicative of the presence of the small molecule. The present invention also provides for and a biosensor comprising nanoparticles coupled to aptamers to provide nanoparticle aptamer-conjugates (NP-aptamer).

Claims35

Dependent claims

A method for the detection of small molecules in a sample comprising the steps:

a) coupling a nanoparticle (NP) or microparticle (MP) to an aptamer specific for the small molecule to be detected to form a NP-aptamer conjugate;
b) contacting the NP-aptamer conjugate with the sample;
and
c) detecting a reduction in the size, a change in surface potential, or a change in mobility of the NP-aptamer conjugate, wherein the reduction or change is indicative of the presence of the small molecule.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

14/765,728Filed 5 Feb 2014

Priority

NZ 6066965 Feb 2013

Published

8 Sep 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 15/115
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: aptamers, i.e. nucleic acids binding a…
C12Q 1/6811
Involving nucleic acids: selection methods for production or design of target specific oligonucleotides or binding molecules
G01N 33/5308
Immunoassay; Biospecific binding assay; Materials therefor: for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
G01N 33/54346
The carrier being characterised by its particulate form: nanoparticles

Novel bio-sensor for the detection of small molecules

Life of this patent

Novel bio-sensor for the detection of small molecules

Life of this patent

Abstract

Claims35