US 2016/0257950 A1 · STABILIZED RNA SOLUTIONS

Abstract

Methods and compositions are described herein for protecting RNA from autocatalytic and divalent cation induced degradation in an aqueous solution.

Dependent claims

A method of protecting RNA from degradation in a solution, said method comprising:

providing a solution comprising:
i. water;
ii. RNA, wherein the RNA is purified from endogenous host proteins of a cell or tissue;
iii. divalent cations;
iv. a DNase enzyme, wherein the DNase enzyme is exogenous to the cell or tissue from which the RNA is purified;
and
v. a phosphorous-containing or a sulfur-containing compound selected from the group consisting of phosphate, glycerol phosphate, glucose phosphate, ribose phosphate, pyrophosphate, polyphosphate, and sulfate;
and
incubating the solution at a temperature of at least 40° C., wherein the incubating the solution at the temperature of at least 40° C. comprises inactivating the DNase enzyme,
wherein the RNA is protected from degradation as compared to an RNA in an aqueous solution that contains the divalent cations but does not contain the phosphorous-containing or sulfur-containing compound.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/063,139Filed 7 Mar 2016

Priority

6 Mar 2015

Published

8 Sep 2016

Term ends

Not estimatedThe source records don’t support an estimate.

C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…
C12N 9/22
Acting on ester bonds: ribonucleases [RNase]; Deoxyribonucleases [DNase]
C12Y 301/00
Hydrolases acting on ester bonds
C12N
Microorganisms or enzymes

Methods and compositions are described herein for protecting RNA from autocatalytic and divalent cation induced degradation in an aqueous solution.

Dependent claims

A method of protecting RNA from degradation in a solution, said method comprising:

providing a solution comprising:
i. water;
ii. RNA, wherein the RNA is purified from endogenous host proteins of a cell or tissue;
iii. divalent cations;
iv. a DNase enzyme, wherein the DNase enzyme is exogenous to the cell or tissue from which the RNA is purified;
and
v. a phosphorous-containing or a sulfur-containing compound selected from the group consisting of phosphate, glycerol phosphate, glucose phosphate, ribose phosphate, pyrophosphate, polyphosphate, and sulfate;
and
incubating the solution at a temperature of at least 40° C., wherein the incubating the solution at the temperature of at least 40° C. comprises inactivating the DNase enzyme,
wherein the RNA is protected from degradation as compared to an RNA in an aqueous solution that contains the divalent cations but does not contain the phosphorous-containing or sulfur-containing compound.

7 more elements

Dependencies are inferred from the claim text.