Modified isoprene synthase
AssigneeAjinomoto Co., Inc.
InventorsKunio NAKATA, Yoshinori Tajima, Uno Tagami, Takashi Oku, Yasuhiro Kashima
Life of this patent
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Abstract
Modified isoprene synthases that have one or more mutations of a given amino acid residue(s) in any amino acid sequence of (a) the amino acid sequence of SEQ ID NO:4, (b) an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO:4, or (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:4, and has an isoprene synthetic activity are useful for preparing isoprene monomer in improved yields.
Claims19
A modified isoprene synthase that has one or more mutations of an amino acid residue corresponding to one or more amino acid residues selected from the following:
- S2, F31, L35, L67, I90, V97, L114, Q123, D124, V125, K127, R128, K130, D131, G134, C137, V144, V187, R202, L224, L247, S248, L249, T257, K259, R264, D265, R266, E269, C286, K292, T298, I300, D301, Y304, D305, E312, F316, E321, V325, I328, C338, L340, C370, E371, C373, E379, S383, K386, I388, A390, Y394, S401, S402, G404, V405, L414, C416, C440, R444, N447, R458, T461, T462, T466, E471, C480, K481, R484, K492, V499, P505, F508, I518, S519, H520, C521, Y523, and G530 in any amino acid sequence of
- (a) the amino acid sequence of SEQ ID NO:4,
- (b) an amino acid sequence having one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence of SEQ ID NO:4, or
- (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:4,
- and has an isoprene synthetic activity.
Dependencies are inferred from the claim text.