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US 2016/0257938 A1Published application
Reprogramming Cells
AssigneeThe Scripps Research Institute
InventorsSaiyong Zhu, Sheng Ding
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Abstract
The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.
Claims19
A method of inducing a non-pluripotent mammalian cell into an induced pluripotent stem cell, the method comprising:
- contacting the non-pluripotent cell with a compound that promotes glycolytic metabolism, a TGFβ receptor/ALK5 inhibitor, and a MEK inhibitor, under conditions sufficient to induce the cell to become a pluripotent stem cell, thereby inducing the non-pluripotent mammalian cell into an induced pluripotent stem cell.
A mixture comprising:
- mammalian cells, a compound that promotes glycolytic metabolism, and one or more of:
- a TGFβ receptor/ALK5 inhibitor,
- a MEK inhibitor, or
- one or more exogenous transcription factors comprising one or more of Oct4, Klf4, and Sox2.
1 more element
Dependencies are inferred from the claim text.