Neuronal stem cell differentiation
OwnerPaul Kemp, Nicholas Alleninventors
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Abstract
The invention relates to at least one novel neural cell differentiation medium for the production of neural cells from at least one precursor cell capable of lineage specific differentiation; a method for neural cell differentiation using said medium; the use of said medium and said method for lineage specific differentiation of at least one precursor cell towards a neural cell fate; and a kit of parts comprising said at least one said neural cell differentiation medium.
Claims32
A neural cell differentiation medium for the production of neural cells from at least one precursor cell capable of lineage specific differentiation, comprising:
- i) at least one cell culture base medium;
- ii) at least one serum-free supplement for neural cell culture;
- iii) a cell-cycle inhibitor;
- iv) at least one Tropomyosin receptor kinase B [TrkB] receptor agonist;
- v) a canonical Wnt pathway agonist;
- vi) ascorbic Acid or vitamin C or a salt or derivative thereof;
- and
- vii) a solution of calcium ions.
-31. (canceled)
A kit for the differentiation of at least one precursor cell capable of lineage specific differentiation into a neural cell comprising:
- a. a neural cell differentiation medium comprising:
- i. at least one cell culture base medium;
- ii. at least one serum-free supplement for neural cell culture;
- iii. a cell-cycle inhibitor;
- iv. at least one Tropomyosin receptor kinase B [TrkB] receptor agonist;
- v. a canonical Wnt pathway agonist;
- vi. ascorbic acid or vitamin C or a salt or derivative thereof;
- vii. a solution of calcium ions;
- viii. a notch signaling inhibitor;
- ix. a CREB (cAMP response element-binding protein) activator;
- x. a γ-aminobutyric acid (GABA) receptor agonist;
- b. a neural cell differentiation medium comprising:
- i. at least one cell culture base medium;
- ii. at least one serum-free supplement for neural cell culture;
- iii. a cell-cycle inhibitor;
- iv. at least one Tropomyosin receptor kinase B [TrkB] receptor agonist;
- v. a canonical Wnt pathway agonist;
- vi. ascorbic acid or vitamin C or a salt or derivative thereof;
- vii. a solution of calcium ions;
- and
- xi) a non-canonical WNT pathway agonist;
- and
- optionally, reagents and instructions pertaining to the use of said neural differentiation mediums.
Dependencies are inferred from the claim text.