US 2016/0256870 A1 · Slip Chip Device and Methods

Abstract

A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Claims86

Dependent claims

Apparatus for determining the quantity of a target protein and other types of biomarkers or analytes present in a sample, the apparatus comprising:

a top plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another;
and
a bottom plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate;
wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the recesses of the top plate communicate with the recesses of the bottom plate so as to form a plurality of rows;
wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels;
and
wherein the recesses in the top plate and the recesses in the bottom plate extend at a 45 degree angle relative to the axis of a row.

4 more elements

A method for determining the quantity of a target protein and other types of biomarkers or analytes present in a sample, the method comprising:

providing an apparatus comprising:
a top plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another;
and
a bottom plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate;
wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the recesses of the top plate communicate with the recesses of the bottom plate so as to form a plurality of rows;
wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels;
and
wherein the recesses in the top plate and the recesses in the bottom plate extend at a 45 degree angle relative to the axis of a row;
binding a protein-specific antibody in at least one recess forming one of the plurality of rows of the top plate;
positioning hydrogen peroxide in a recess adjacent to the row containing the protein-specific antibody;
positioning ink in a recess in a row adjacent to the plurality of channels;
positioning a sample in the at least one recess containing the protein-specific antibody;
positioning a catalase in the at least one recess containing the protein-specific antibody and the sample;
sliding one of the top plate and the bottom plate relative to the other of the top plate and the bottom plate so as to form the plurality of columns, with each column being in communication with one of the plurality of channels;
and
determining the quantity of the target protein and other biomarker or other molecular analyte present in the sample by detecting the longitudinal position of the ink contained in the plurality of channels.

13 more elements

A method for determining the quantity of a target analyte present in a sample, the method comprising:

providing an apparatus comprising:
a top plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another;
and
a bottom plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate;
wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the recesses of the top plate communicate with the recesses of the bottom plate so as to form a plurality of rows;
wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels;
and
wherein the recesses in the top plate and the recesses in the bottom plate extend at a 45 degree angle relative to the axis of a row;
binding a capture agent in at least one recess forming one of the plurality of rows of the top plate, introducing a sample into the at least one recess so that an analyte contained in the sample is bound to the capture agent, and binding a probe to the bound analyte;
and positioning a reagent in a recess adjacent to the row containing the capture agent, bound analyte and bound probe;
and positioning ink in a recess in a row adjacent to the plurality of channels;
sliding one of the top plate and the bottom plate relative to the other of the top plate and the bottom plate so as to form the plurality of columns, with each column being in communication with one of the plurality of channels;
and
determining the quantity of the analyte present in the sample by detecting the longitudinal position of the ink contained in the plurality of channels.

11 more elements