Prrsv gp5 based compositions and methods
AssigneeMJ Biologics, Inc.
InventorBYOUNG-KWAN KIM
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Abstract
The disclosure includes compositions and methods for the production of an immune response against porcine reproductive and respiratory syndrome (PRRS) virus, or PRRSV. The disclosure is based in part on the use of two or more peptide domains, each with a different sequence, from the PRRSV GP5 protein ectodomain. Compositions and methods comprising polypeptides containing the two or more domains, or nucleic acids encoding them, are described.
Claims25
An immunogenic composition, comprising:
- a first separated PRRSV GP5 antigenic polypeptide comprising a first HV-2 hypervariable region;
- and
- a second separated PRRSV GP5 antigenic polypeptide comprising a second HV-2 hypervariable region, the second HV-2 region selected to be different from the first HV-2 hypervariable region.
An immunogenic composition, comprising a first GP5 antigenic polypeptide domain having a length from 14 to 201 amino acids comprising a first HV-2 hypervariable region having a length of about 8 amino acids, and a second GP5 antigenic polypeptide domain having a length from 14 to 201 amino acids comprising a second HV-2 hypervariable region having a length of about 8 amino acids, the second HV-2 region selected to be different from the first HV-2 hypervariable region, the immunogenic composition being substantially free of intact viral particles.
A method of making an immunogenic composition, comprising:
- identifying or selecting a first PRRSV GP5 antigenic polypeptide comprising a first HV-2 hypervariable region;
- purposefully selecting a second PRRSV GP5 antigenic polypeptide that has a second HV-2 hypervariable region different from the first HV-2 hypervariable region;
- and
- combining the first and second PRRSV GP5 antigenic polypeptides to form the immunogenic composition.
Dependencies are inferred from the claim text.