Biologic composition and method of manufacture

A method of making a biological composition comprises the steps of:

• collecting, recovering and processing bone marrow from a cadaver donor;
• mechanically separating cellular and non-cellular components of bone marrow from cadaverous bone;
• concentrating by centrifugation and filtering;
• separation by density gradient centrifugation;
• collecting cells or non-cellular components or combinations thereof of predetermined density;
• washing the cells or non-cellular components or combinations thereof to create a mixture;
• quantifying cell concentration not to exclude zero;
• suspending to a predetermined concentration in a polyampholyte cryoprotectant;
• freezing the mixture at a predetermined controlled rate;
• and
• packaging a bone blend having particles in the size range of 100 to 300 um of demineralized cortical bone, mineralized cortical bone and mineralized cancellous bone either within the mixture or separate.