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US 2016/0254130 A1Published application
MS/MS Analysis Using ECD or ETD Fragmentation
AssigneeMicromass UK Limited
InventorJeffery Mark Brown
Life of this patent
Estimating the term…
Abstract
A method of mass spectrometry is disclosed comprising: providing supercharged analyte ions; and supplying electrons or reagent ions to said analyte ions so as to transfer charge from said reagent ions or electrons to said analyte ions, said transfer of charge causing at least some of said analyte ions to dissociate. The charge transfer step is performed at a relatively high pressure and preferably substantially at atmospheric pressure.
Claims43
A method of mass spectrometry comprising:
- (a) providing supercharged analyte ions;
- and
- (b) supplying electrons or reagent ions to said analyte ions so as to transfer charge from said reagent ions or electrons to said analyte ions, said transfer of charge causing at least some of said analyte ions to dissociate;
- wherein step (b) is performed at a pressure selected from the group of >0.1 mbar;
- >1 mbar;
- >5 mbar;
- >10 mbar;
- >100 mbar;
- about 1 bar;
- or substantially at atmospheric pressure.
7 more elements
A method of mass spectrometry comprising:
- providing a mixture of different supercharged analyte ions;
- supplying electrons or reagent ions to said mixture of different analyte ions in a region at a pressure selected from the group of >0.1 mbar;
- >1 mbar;
- >5 mbar;
- >10 mbar;
- >100 mbar;
- about 1 bar;
- or substantially at atmospheric pressure so as to transfer charge from said reagent ions or electrons to said analyte molecules or ions, said transfer of charge causing at least some of said analyte molecules or analyte ions to dissociate and others of said analyte molecules or analyte ions not to dissociate but to form intermediate ions of altered charge;
- isolating at least some of said intermediate ions from other ions;
- exciting at least some of the isolated intermediate ions so as to cause them to dissociate into daughter ions;
- analysing at least some of the intermediate ions and at least some of their daughter ions so as to associate at least some of the intermediate ions with their daughter ions;
- and
- identifying intermediate ions from their daughter ions.
10 more elements
A method of mass spectrometry comprising:
- (a) providing analyte molecules or analyte ions using a MALDI ion source;
- (b) supplying electrons or reagent ions to said analyte molecules or analyte ions so as to transfer charge from said reagent ions or electrons to said analyte molecules or ions, said transfer of charge causing at least some of said analyte molecules or analyte ions to dissociate and others of said analyte molecules or analyte ions not to dissociate but to form intermediate ions of altered charge;
- (c) isolating at least some of said intermediate ions from other ions;
- (d) exciting at least some of the isolated intermediate ions so as to cause them to dissociate into daughter ions;
- and
- (e) mass analysing at least some of said intermediate ions and/or mass analysing at least some of said daughter ions.
3 more elements
A method of mass spectrometry comprising:
- (a) providing a mixture of different analyte molecules or analyte ions using a MALDI ion source;
- (b) supplying electrons or reagent ions to said mixture of different analyte molecules or analyte ions so as to transfer charge from said reagent ions or electrons to said analyte molecules or ions, said transfer of charge causing at least some of said analyte molecules or analyte ions to dissociate and others of said analyte molecules or analyte ions not to dissociate but to form intermediate ions of altered charge;
- (c) isolating at least some of said intermediate ions from other ions;
- (d) exciting at least some of the isolated intermediate ions so as to cause them to dissociate into daughter ions;
- (e) analysing at least some of the intermediate ions and at least some of their daughter ions so as to associate at least some of the intermediate ions with their daughter ions;
- and
- (f) identifying intermediate ions from their daughter ions.
4 more elements
A method of mass spectrometry or ion mobility spectrometry comprising:
- directing laser light at analyte on a MALDI sample plate so as to ionise the analyte and form multiply charged analyte ions;
- supplying the analyte ions to a reaction region that is at a pressure selected from the group of >0.1 mbar;
- >1 mbar;
- >5 mbar;
- >10 mbar;
- >100 mbar;
- about 1 bar;
- or substantially at atmospheric pressure;
- providing free electrons or reagent ions in the reaction region;
- reacting the electrons or reagent ions with the analyte ions in the reaction region so as to cause ECD or ETD of the analyte ions and thereby form fragment ions;
- and
- analysing the fragment ions in a mass analyser or ion mobility spectrometer.
9 more elements
A mass spectrometer or ion mobility spectrometer comprising:
- a laser;
- a MALDI sample plate;
- a reaction region having means to maintain the reaction region at a pressure selected from the group of >0.1 mbar;
- >1 mbar;
- >5 mbar;
- >10 mbar;
- >100 mbar;
- about 1 bar;
- or substantially at atmospheric pressure;
- means for directing laser light at the MALDI sample plate for ionising analyte thereon to form multiply charged analyte ions;
- means for supplying the analyte ions from the sample plate to the reaction region;
- means for providing free electrons or reagent ions in the reaction region such that, in use, the electrons or reagent ions react with the analyte ions in the reaction region so as to cause ECD or ETD of the analyte ions and thereby form fragment ions;
- and
- a mass analyser or ion mobility analyser for analysing the fragment ions.
11 more elements
Dependencies are inferred from the claim text.