Method for detecting microorganism having fluorescent characteristics with respect to specific wavelength

A method for determining a number of individuals of microorganisms included in a sample using an optical apparatus, comprising a light source unit for supplying light to a sample comprising microorganisms which reflect only light of a specific wavelength range and have a fluorescent characteristic and a detection unit comprising an image acquisition unit for generating an image by collecting the light reflected by the microorganisms and an image processing unit for processing the generated image, the method comprising steps of:

• (a) collecting, by the image acquisition unit, light radiated by microorganisms which radiate only light of a specific wavelength range and are included in the sample;
• (b) generating, by the image acquisition unit, an image of the collected image;
• (c) displaying, by the image processing unit, the image generated by the image acquisition unit on a screen, analyzing each of pixels forming the screen on which the image has been displayed, and classifying a plurality of pixels which belongs to the pixels included in the screen and continuously emit light to be a single object;
• and
• (d) calculating, by the image processing unit, a total number of objects classified in the screen.