Biomarkers of Alzheimer's Disease Progression

2:

• A method as claimed in claim 1, wherein said index is calculated to express the information content of a ratio (Tau-A′/Tau-C′), wherein Tau-A′ is said first level and Tau-C′ is said second level, each being expressed as a coefficient of the Normal Reference Mean of the measurement.

3:

• A method as claimed in claim 1, wherein said sample is a sample of blood, serum, plasma, CSF, saliva, sputum, tissues, extracts of tissue, BALF fluid.

4:

• A method for determining which patients should be enrolled in a clinical trial of a medicament for treatment or prevention of Alzheimer's disease comprising for each candidate patient conducting a method of assay as claimed in claim 1, and selecting for enrollment patients having an index predictive of a change in ADAS-Cog11 of at least three points over 52 weeks.

5:

• A method as claimed in claim 4, wherein patients are selected having an index predictive of a change in ADAS-Cog11 of at least three points over 40 weeks.

6:

• A method as claimed in claim 4, wherein patients are selected having an index predictive of a change in ADAS-Cog11 of at least three points over 28 weeks.

7:

• A method as claimed in claim 4, wherein patients are selected having an index predictive of a change in ADAS-Cog11 of at least six points over the stated number of weeks.

8:

• A method for determining which patients should be enrolled in a clinical trial of a medicament for treatment or prevention of Alzheimer's disease comprising for each candidate patient conducting a method of assay as claimed in claim 2, and selecting for enrolment patients having an index corresponding to a (Tau-A′/Tau-C′) ratio of greater than 1.5.

9:

• A method as claimed in claim 8, comprising selecting for enrolment patients having a said index corresponding to a (Tau-A′/Tau-C′) ratio of greater than 2.0.

10:

• A method as claimed in claim 9, wherein the selected patients have a (Tau-A′/Tau-C′) ratio of greater than 2.5.

11:

• A method for determining the efficacy of a candidate intervention for treatment or prevention of Alzheimer's disease comprising applying said intervention to a first panel of patients and applying a comparator intervention to a second panel of patients, carrying out for each patient an assay as claimed in claim 1, and comparing the values for said index obtained for said first panel of patients with values for said index obtained for said second panel of patients, and determining whether the candidate intervention shows a superior effect in modulating said index values in comparison to the comparator intervention.

12:

• A method for selecting patients for treatment with an intervention for treatment or prevention of Alzheimer's disease comprising conducting a method of assay as claimed in claim 1 and selecting patients for treatment based on a resulting index predictive of a change in ADAS-Cog11 or a (Tau-A′/Tau-C′) ratio.

13:

• A method as claimed in claim 12, wherein the index predictive of a change in ADAS-Cog11 is at least three points over 52 weeks.

14:

• A method as claimed in claim 12, wherein the index predictive of a change in ADAS-Cog11 is at least three points over 40 weeks.

15:

• A method as claimed in claim 12, wherein the index predictive of a change in ADAS-Cog11 is at least three points over 28 weeks.

16:

• A method as claimed in claim 12, wherein the index predictive of a change in ADAS-Cog11 is at least six points over any of 52 weeks, 40 weeks or 28 weeks.

17:

• A method as claimed in claim 12, wherein the (Tau-A′/Tau-C′) ratio is greater than 1.5.

18:

• A method as claimed in claim 12, wherein the (Tau-A′/Tau-C′) ratio is greater than 2.0.

19:

• A method as claimed in claim 12, wherein the (Tau-A′/Tau-C′) ratio is greater than 2.5.