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US 2016/0252522 A1Published application
Method for preparing peptide fragments, kit for preparing peptide fragments to be used therein, and analysis method
AssigneeShimadzu Corporation
InventorsTakashi SHIMADA, Noriko FUKAO, Chikage AOKI, Taka-Aki SATO
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Abstract
A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.
Claims27
A method of proteolyzing a protein, comprising:
- immobilizing a protein in at least one pore of a porous body;
- and
- contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.
A kit for proteolyzing a protein, comprising:
- at least one porous body having a plurality of pores in which a protein is to be immobilized;
- and
- a plurality of particles having an average particle diameter larger than an average pore diameter of the pores,
- wherein the particles have a surface on which a protease has been immobilized or to be immobilized.
1 more element
Dependencies are inferred from the claim text.