Method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule as well as kits, compositions and uses relating thereto

An in vitro method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule capable of binding with its binding site to the analyte, the method comprising the steps:

• (i) contacting a sample comprising the analyte and the binding molecule with
• a trapping molecule directed against the binding site of the binding molecule and
• a detection molecule capable of forming a complex with the analyte, and
• (ii) detecting the detection molecule-analyte complex, thereby determining the total amount and/or concentration of the analyte,
• wherein the detection molecule is different from the binding molecule, and
• wherein the analyte is different from the trapping molecule, and
• wherein the detection molecule is only capable of forming a complex with the analyte
• when the analyte is not bound by the binding molecule.