Endometriosis Classifier
AssigneeThe Regents of the University of California
InventorsLinda C. Giudice, Juan C. Irwin, John S. Tamaresis
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Abstract
The present disclosure provides methods and compositions that are useful for diagnosing the presence or absence of endometriosis and the severity of endometriosis in a subject. The methods and compositions are also useful for distinguishing endometriosis from other uterine or pelvic pathologies in a subject. Also described are sets of genes whose expression levels in a biological sample are diagnostic for endometriosis, and compositions useful for diagnosis, prognosis, and/or treatment of endometriosis.
Claims21
A method for diagnosing endometriosis, comprising:
- determining the expression level of at least one set of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15 in a tissue sample comprising endometrial cells from a subject;
- associating the expression level with the presence and severity of endometriosis;
- and
- providing a diagnosis of the presence, absence or severity of endometriosis based on the association.
A kit for diagnosing endometriosis, the kit comprising a plurality of oligonucleotides that specifically hybridize to mRNA, or a complement thereof, expressed by a set of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15, or any combination thereof.
A kit for diagnosing the presence of endometriosis, the kit comprising a set of oligonucleotides that specifically hybridize to mRNA, or a complement thereof, expressed by the set of genes in Table 7, the set of genes in Table 8, the set of genes in Table 10, the set of genes in Table 11, the set of genes in Table 13, or the set of genes in Table 14.
A kit for diagnosing the severity of endometriosis, the kit comprising a set of oligonucleotides that specifically hybridize to mRNA, or a complement thereof, expressed by the set of genes in Table 9 the set of genes in Table 12, or the set of genes in Table 15.
A kit for diagnosing endometriosis comprising a set of probes for detecting nucleic acids or proteins expressed by a plurality of the genes in Tables 7-15.
A microarray comprising a set of oligonucleotides that specifically hybridize to mRNA expressed by one or more sets of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15, or any combination thereof.
A computer implemented method for diagnosing endometriosis, comprising:
- (i) receiving the expression data of at least one set of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15;
- and
- (ii) associating the expression data of the at least one set of genes with the presence, absence or severity of endometriosis, thereby diagnosing endometriosis.
A computer product comprising a non-transitory computer readable medium storing a plurality of instructions for controlling a processor to perform an operation of one or more of the following steps:
- (i) receiving the expression data of at least one set of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, and/or Table 15;
- and
- (ii) associating the expression data of the at least one set of genes with the presence, absence or severity of endometriosis.
A system comprising:
- the computer product of claim 20;
- and
- one or more processors for executing instructions stored on the computer readable medium.
A method of diagnosing endometriosis, the method comprising:
- i) obtaining a tissue sample comprising endometrial cells from a subject suspected of suffering from endometriosis;
- ii) determining the expression level of at least one set of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15, in the tissue sample;
- iii) comparing the expression level with the expression level of the at least one set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15, in a tissue sample from a control subject not having endometriosis;
- iv) providing a diagnosis of the presence, absence or severity of endometriosis if the expression level of the set of genes in the tissue sample from the subject suspected of suffering from endometriosis is statistically different from the expression level in the tissue sample from the control subject.
A method for detecting the expression of genes in a tissue sample comprising endometrial cells or tissue, comprising:
- detecting the expression level of at least one set of genes, the set of genes comprising the genes in Table 7, Table 8, Table 9, Table 10, Table 11, Table 12, Table 13, Table 14, or Table 15, in a tissue sample comprising endometrial cells from a subject.
Dependencies are inferred from the claim text.