Multiplex slide plate device and operation method thereof

A multiplex slide plate device, comprising:

• a slide plate having a plurality of reaction vessels, wherein the reaction vessels are arranged in an anay, and each of the reaction vessels has an opening portion and a bottom portion;
• and
• a sacrificial layer having a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other, and the sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion;
• wherein a sample solution is injected to the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, and the sample solution loads into each of the reaction vessels while flowing through the main channel.

An operation method of a multiplex slide plate device, including:

• assembling the multiplex slide plate device including a slide plate, a sacrificial layer and a housing for accommodating the slide plate and the sacrificial layer, and the slide plate has a plurality of reaction vessels arranged in an array, wherein each of the reaction vessels has an opening portion and a bottom portion, and the sacrificial layer has a microfluidic channel composed of an injection channel, a main channel and a distal channel connected to each other, wherein the main channel faces the opening portion;
• injecting a sample solution to the injection channel through an injection hole of the housing, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel;
• injecting an oil to the injection channel through the injection hole of the housing, such that the oil flows from the injection channel through the main channel to the distal channel, wherein the oil removes the sample solution which is not loaded into the reaction vessels while flowing through the main channel;
• and
• heating the sacrificial layer to melt, and the melted sacrificial layer is mixed with the oil.