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US 2016/0251697 A1Published application
Methods of identifying multiple epitopes in cells
AssigneeRoche Sequencing Solutions, Inc.
InventorGarry P. NOLAN
Life of this patent
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Abstract
The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
Claims26
A method for identifying whether a plurality of targets are in a plurality of cells comprising:
- binding to the targets a plurality of tags, wherein a tag is a nucleic acid comprising a code that represents the target identity;
- and adding multiple APSs to the tags to represent the identity of the cell in which the tags are binding.
(canceled)
The method of claim 15, wherein the tag comprises multiple APSs, an ESB and a UBA.
A composition comprising:
- a) a first target molecule,
- b) a first unique binding agent (UBA) specific for the first target molecule,
- c) a first linkable UBA-dependent epitope specific barcode (ESB), and
- d) a plurality of ordered assayable polymer subunit (APS), wherein the order of APSs is detectable.
1 more element
25-312. (canceled)
Dependencies are inferred from the claim text.