Pollen preferred promoters and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsAndrew Mark Cigan, Shai Joshua Lawit
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Abstract
Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
Claims27
A method for selectively expressing gene products in plant male tissue, comprising
- a) transforming a plant using an isolated nucleic acid molecule comprising a polynucleotide selected from the group comprising:
- (i) a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:53, 54, 55 and 56;
- (ii) a nucleotide sequence comprising a fragment or variant of the nucleotide sequence of SEQ ID NO:
- 53, 54, 55 and 56, wherein the sequence initiates transcription in a plant cell;
- (iii) a polynucleotide which is complementary to the polynucleotide of (a) or (b),
- b) growing the plant under normal plant growing conditions, where the polynucleotide encodes a pollen preferred promoter which drives pollen specific expression.
A method for expressing a polynucleotide in a plant or a plant cell, said method comprising introducing into the plant or the plant cell an expression cassette comprising a promoter operably linked to a heterologous polynucleotide of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:53, 54, 55 and 56;
- (b) a nucleotide sequence comprising a fragment or variant of the nucleotide sequence of SEQ ID NO:
- 53, 54, 55 and 56, wherein the sequence initiates transcription in a plant cell;
- (c) a nucleotide sequence which is complementary to (a) or (b)
- where the nucleotide sequence encodes a pollen preferred promoter which drives pollen specific expression.
A method for expressing a polynucleotide preferentially in pollen cells of a plant, said method comprising introducing into a plant cell an expression cassette and regenerating a plant from said plant cell, said plant having stably incorporated into its genome the expression cassette, said expression cassette comprising a promoter operably linked to a heterologous polynucleotide of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:
- 53, 54, 55 and 56;
- (b) a nucleotide sequence comprising a fragment or variant of the nucleotide sequence of SEQ ID NO:
- 53, 54, 55 and 56, wherein the sequence initiates transcription in a plant cell;
- (c) a nucleotide sequence which is complementary to (a) or (b)
- where the polynucleotide encodes a pollen preferred promoter which drives pollen specific expression.
Dependencies are inferred from the claim text.