Novel polypeptides and uses thereof
AssigneeInvista North America S.A.R.L.
InventorsADRIANA L. BOTES, Nadia Kadi
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Abstract
The present disclosure provides novel polypeptides with improved 3-buten-2-ol dehydratase activity, polypeptides with improved linalool dehydratase activity, and polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene. Methods of making and using the polypeptides are also provided.
Claims67
A polypeptide comprising an amino acid sequence with at least 90% amino acid sequence homology to SEQ ID NO:11 or SEQ ID NO:51, wherein said amino acid sequence comprises at least 1-3 alteration(s) relative to SEQ ID NO:11 or SEQ ID NO:51 independently selected from:
- a substitution of the amino acid that occupies position 230 with a different amino acid selected from E and equivalent amino acids;
- a substitution of the amino acid that occupies position 366 with a different amino acid selected from V and equivalent amino acids;
- and
- a substitution of the amino acid that occupies position 168 with a different amino acid selected from D and equivalent amino acids;
- wherein the polypeptide optionally comprises at least one mutation selected from A230E, L366V, and S168D;
- preferably comprises the amino acid sequence of the A230E variant of SEQ ID NO:42, the amino acid sequence of the L366V variant of SEQ ID NO:45, or the amino acid sequence of the S168D variant of SEQ ID NO:48.
-18. (canceled)
-24. (canceled)
A polypeptide comprising an amino acid sequence with at least 90% amino acid sequence homology to SEQ ID NO:11 or SEQ ID NO:51, wherein said amino acid sequence comprises at least 1-3 alteration(s) relative to SEQ ID NO:11 or SEQ ID NO:51 independently selected from:
- a substitution of the amino acid that occupies position 58 with a different amino acid selected from R and equivalent amino acids;
- a substitution of the amino acid that occupies position 83 with a different amino acid selected from A and equivalent amino acids;
- and
- a substitution of the amino acid that occupies position 252 with a different amino acid selected from A and equivalent amino acids.
-42. (canceled)
-49. (canceled)
A polypeptide comprising an amino acid sequence with at least 90% amino acid sequence homology to SEQ ID NO:11 or SEQ ID NO:51, wherein said amino acid sequence comprises at least 1-4 alteration(s) relative to SEQ ID NO:11 or SEQ ID NO:51 independently selected from:
- a substitution of the amino acid that occupies position 83 with a different amino acid selected from A and equivalent amino acids;
- a substitution of the amino acid that occupies position 169 with a different amino acid selected from S, G, H, D and equivalent amino acids;
- a substitution of the amino acid that occupies position 186 with a different amino acid selected from C, M and equivalent amino acids;
- and
- a substitution of the amino acid that occupies position 359 with a different amino acid selected from S, L and equivalent amino acids.
-83. (canceled)
-89. (canceled)
-125. (canceled)
-129. (canceled)
(canceled)
-135. (canceled)
-142. (canceled)
A composition comprising (i) 3-buten-2-ol and a means for producing 1,3-butadiene, and/or (ii) linalool and a means for producing myrcene.
A composition comprising (i) a substrate and a means for enzymatically producing 1,3-butadiene from said substrate;
- and/or (i) a substrate and a means for enzymatically producing myrcene from said substrate.
A method of:
- (i) producing 1,3-butadiene comprising:
- a step for enzymatically converting 3-buten-2-ol to 1,3-butadiene;
- and
- measuring and/or harvesting the 1,3-butadiene thereby produced;
- and/or
- (ii) producing myrcene comprising:
- a step for enzymatically converting linalool to myrcene;
- and
- measuring and/or harvesting the myrcene thereby produced.
-169. (canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.