Fusion proteins and combination vaccines comprising haemophilus influenzae protein e and pilin a
AssigneeGlaxosmithkline Biologicals SA
InventorsNormand BLAIS, Steve LABBE, Jan POOLMAN
Life of this patent
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Abstract
The present invention relates to compositions comprising Haemophilus influenzae Protein E and Pilin A. More particularly, the present application relates to fusion proteins and immunogenic compositions comprising Protein E and PilA, vaccines comprising such immunogenic compositions and therapeutic uses of the same.
Claims10
A method for the treatment of otitis media caused wholly or in part by non-typeable Haemophilus influenzae in a subject in need thereof comprising administering to said subject a therapeutically effective amount of an immunogenic composition comprising the fusion protein of SEQ ID NO. 177
- (QIQKAEQN DVKLAPPTDV RSGYIRLVKN VNYYIDSESI
- WVDNQEPQIV HFDAVVNLDK GLYVYPEPKR YARSVRQYKI
- LNCANYHLTQ VRTDFYDEFW GQGLRAAPKK QKKHTLSLTP
- DTTLYNAAQI ICANYGEAFS VDKKGGTKKA AVSELLQASA
- PYKADVELCV YSTNETTNCT GGKNGIAADI TTAKGYVKSV
- TTSNGAITVK GDGTLANMEY ILQATGNAAT GVTWTTTCKG
- TDASLFPANF CGSVTQ).
A method for the treatment of otitis media caused wholly or in part by non-typeable Haemophilus influenzae in a subject in need thereof comprising administering to said subject a therapeutically effective amount of an immunogenic composition comprising the fusion protein of SEQ ID NO. 219
- (IQKAEQND VKLAPPTDVR SGYIRLVKNV NYYIDSESIW
- VDNQEPQIVH FDAVVNLDKG LYVYPEPKRY ARSVRQYKIL
- NCANYHLTQV RTDFYDEFWG QGLRAAPKKQ KKHTLSLTPD
- TTLYNAAQII CANYGEAFSV DKKGGTKKAA VSELLQASAP
- YKADVELCVY STNETTNCTG GKNGIAADIT TAKGYVKSVT
- TSNGAITVKG DGTLANMEYI LQATGNAATG VTWTTTCKGT
- DASLFPANFC GSVTQ).
A method for the treatment of pneumonia caused wholly or in part by non-typeable Haemophilus influenzae in a subject in need thereof comprising administering to said subject a therapeutically effective amount of an immunogenic composition comprising the fusion protein of SEQ ID NO. 177
- (QIQKAEQN DVKLAPPTDV RSGYIRLVKN VNYYIDSESI
- WVDNQEPQIV HFDAVVNLDK GLYVYPEPKR YARSVRQYKI
- LNCANYHLTQ VRTDFYDEFW GQGLRAAPKK QKKHTLSLTP
- DTTLYNAAQI ICANYGEAFS VDKKGGTKKA AVSELLQASA
- PYKADVELCV YSTNETTNCT GGKNGIAADI TTAKGYVKSV
- TTSNGAITVK GDGTLANMEY ILQATGNAAT GVTWTTTCKG
- TDASLFPANF CGSVTQ).
A method for the treatment of pneumonia caused wholly or in part by non-typeable Haemophilus influenzae in a subject in need thereof comprising administering to said subject a therapeutically effective amount of an immunogenic composition comprising the fusion protein of SEQ ID NO. 219
- (IQKAEQND VKLAPPTDVR SGYIRLVKNV NYYIDSESIW
- VDNQEPQIVH FDAVVNLDKG LYVYPEPKRY ARSVRQYKIL
- NCANYHLTQV RTDFYDEFWG QGLRAAPKKQ KKHTLSLTPD
- TTLYNAAQII CANYGEAFSV DKKGGTKKAA VSELLQASAP
- YKADVELCVY STNETTNCTG GKNGIAADIT TAKGYVKSVT
- TSNGAITVKG DGTLANMEYI LQATGNAATG VTWTTTCKGT
- DASLFPANFC GSVTQ).
A method for the treatment of a H. influenzae infection or disease in a subject in need thereof, said method comprising administering to said subject a therapeutically effective amount of an immunogenic composition comprising the fusion protein of SEQ ID NO. 177
- (QIQKAEQN DVKLAPPTDV RSGYIRLVKN VNYYIDSESI
- WVDNQEPQIV HFDAVVNLDK GLYVYPEPKR YARSVRQYKI
- LNCANYHLTQ VRTDFYDEFW GQGLRAAPKK QKKHTLSLTP
- DTTLYNAAQI ICANYGEAFS VDKKGGTKKA AVSELLQASA
- PYKADVELCV YSTNETTNCT GGKNGIAADI TTAKGYVKSV
- TTSNGAITVK GDGTLANMEY ILQATGNAAT GVTWTTTCKG
- TDASLFPANF CGSVTQ).
A method for the treatment of a H. influenzae infection or disease in a subject in need thereof, said method comprising administering to said subject a therapeutically effective amount of an immunogenic composition comprising the fusion protein of SEQ ID NO. 219
- (IQKAEQND VKLAPPTDVR SGYIRLVKNV NYYIDSESIW
- VDNQEPQIVH FDAVVNLDKG LYVYPEPKRY ARSVRQYKIL
- NCANYHLTQV RTDFYDEFWG QGLRAAPKKQ KKHTLSLTPD
- TTLYNAAQII CANYGEAFSV DKKGGTKKAA VSELLQASAP
- YKADVELCVY STNETTNCTG GKNGIAADIT TAKGYVKSVT
- TSNGAITVKG DGTLANMEYI LQATGNAATG VTWTTTCKGT
- DASLFPANFC GSVTQ).
The method of claim 7, wherein the H. influenzae infection or disease is an NTHi infection or disease.
A process for producing periplasmic expression of a fusion protein wherein the process comprises inducing expression of proteins containing a signal peptide wherein the fusion protein is the fusion protein of SEQ ID NO. 148 wherein the signal peptide has been removed, SEQ ID NO. 177
- (QIQKAEQN DVKLAPPTDV RSGYIRLVKN VNYYIDSESI
- WVDNQEPQIV HFDAVVNLDK GLYVYPEPKR YARSVRQYKI
- LNCANYHLTQ VRTDFYDEFW GQGLRAAPKK QKKHTLSLTP
- DTTLYNAAQI ICANYGEAFS VDKKGGTKKA AVSELLQASA
- PYKADVELCV YSTNETTNCT GGKNGIAADI TTAKGYVKSV
- TTSNGAITVK GDGTLANMEY ILQATGNAAT GVTWTTTCKG
- TDASLFPANF CGSVTQ).
A process for producing periplasmic expression of a fusion protein wherein the process comprises inducing expression of proteins containing a signal peptide wherein the fusion protein is the fusion protein of SEQ ID NO. 194 wherein the signal peptide has been removed, SEQ ID NO. 219
- (IQKAEQND VKLAPPTDVR SGYIRLVKNV NYYIDSESIW
- VDNQEPQIVH FDAVVNLDKG LYVYPEPKRY ARSVRQYKIL
- NCANYHLTQV RTDFYDEFWG QGLRAAPKKQ KKHTLSLTPD
- TTLYNAAQII CANYGEAFSV DKKGGTKKAA VSELLQASAP
- YKADVELCVY STNETTNCTG GKNGIAADIT TAKGYVKSVT
- TSNGAITVKG DGTLANMEYI LQATGNAATG VTWTTTCKGT
- DASLFPANFC GSVTQ).
Dependencies are inferred from the claim text.