Vascular Closure Devices and Methods Providing Hemostatic Enhancement

A method for closing a blood vessel puncture site disposed at a distal end of a tissue tract, the method comprising:

• introducing a closure device through the tissue tract, wherein the closure device comprises an insoluble chemical and/or biological agent immobilized on a region of the closure device and a sealing member disposed over the region, wherein the insoluble chemical and/or biological agent, the region of the closure device the insoluble chemical and/or biological agent is immobilized on, and the sealing member are concurrently introduced through the tissue tract;
• deploying an expansible member on a distal end of the closure device within the blood vessel, wherein the region of the closure device the insoluble chemical and/or biological agent is immobilized on is proximal of the expansible member;
• deploying a coil spring disposed proximal the expansible member into the tissue tract;
• tensioning the coil spring to apply a tension to the expansible member so that the expansible member is seated against the puncture site;
• retracting the sealing member relative to the region of the closure device the insoluble chemical and/or biological agent is immobilized on to expose blood in the tissue tract to the insoluble chemical and/or biological agent, wherein said insoluble chemical and/or biological agent promotes hemostasis in the tissue tract and remains immobilized on the device after exposure to the blood;
• and
• collapsing the expansible member and removing the closure device so that the region of the closure device the insoluble chemical and/or biological agent is immobilized on is removed from the tissue tract,
• wherein the insoluble chemical and/or biological agent has a positive electrical charge which attracts platelets.