Method for pooling hepatocytes
AssigneeTriangle Research Labs, LLC
InventorsMatthew Sherman, Robert Kaiser
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Abstract
The present invention relates to a novel method for the preparation of a pooled or mixed population of cryopreserved cells (e.g. hepatocytes). In particular, the invention entails the rapid thaw of cells (e.g. hepatocytes), donated from a single individual, which are mixed to create a heterogeneous population and then cryopreserved. The invention also concerns preparations of multi-cryopreserved cells to increase viability prior to immediate use. The process entails reducing exposure to chemical and physical stresses to increase the resultant number of viable cells.
Claims13
A method for cryopreserving hepatocytes from multiple sources comprising the steps of:
- A) thawing hepatocytes from a plurality of sources;
- B) pooling the hepatocytes from the plurality of sources into a preservation solution;
- C) centrifuging the pooled hepatocytes to cause pelleting;
- D) removing the preservation solution;
- E) combining the viable and non-viable pelleted hepatocytes with a cryopreservative;
- F) distributing the pooled hepatocytes into vials
- G) cryopreserving the hepatocytes in the vials
Dependencies are inferred from the claim text.