Antigen receptor screening assay
AssigneeX-Body, Inc.
InventorRichard W. Wagner
Life of this patent
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Abstract
The present invention provides methods for the identification of an antigen receptor (e.g., an antibody) that specifically binds to an antigen of interest. Generally, this involves contacting a plurality of antigen receptor-expressing cells with an antigen of interest; measuring the level of activated adhesion molecules on the surface of the antigen receptor-expressing cells; and, identifying from the plurality of antigen receptor-expressing cells an antigen receptor-expressing cell that exhibits an increased amount of activated adhesion molecules on the cell surface.
Claims27
A method for identifying an antigen receptor that specifically binds to an antigen of interest, the method comprising:
- (a) contacting a plurality of antigen receptor-expressing cells with the antigen;
- (b) measuring the amount of activated adhesion molecules on the surface of the antigen receptor-expressing cells in the presence and absence of the antigen;
- and
- (c) identifying from the plurality of antigen receptor-expressing cells an antigen receptor-expressing cell that specifically binds to the antigen, wherein an increase in the amount of activated adhesion molecules on the surface of an antigen receptor-expressing cell in the presence of the antigen relative to a suitable control is indicative of the binding of the antigen to the antigen receptor-expressing cell, thereby identifying an antigen receptor that specifically binds to an antigen of interest.
The method of any of the preceding claims, wherein the adhesion molecules are integrins.
The method of any of the preceding claims, wherein the amount of activated adhesion molecules is measured by measuring the binding of the antigen receptor-expressing cells to an extracellular matrix protein or to an antibody that binds to activated adhesion molecules but not to quiescent adhesion molecules.
The method of any of the preceding claims, wherein the antigen receptor is a B-cell receptor.
The method of any of the preceding claim, wherein the antigen receptor-expressing cells are B-cells or hybridoma cells.
A method for producing an antigen receptor that specifically binds to an antigen of interest, the method comprising:
- (a) identifying an antigen receptor according to the method of any one of the preceding claims;
- and,
- (b) expressing the antigen receptor, or an antigen-binding portion thereof.
Dependencies are inferred from the claim text.