Cell analysis using dynamic biophysical methods
AssigneeBar-Ilan University
InventorsYuval GARINI, Irena BRONSHTEIN BERGER, Eldad KEPTEN
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Abstract
A method for identifying the expression or activity of a nuclear protein in a cell, the method comprising: analyzing multiple images of one or more labeled genetic entities; determining from said analysis, a motile property of said one or more labeled genetic entities; and identifying an expression or activity of a nuclear protein of said cell associated with said motile property.
Claims25
A method for identifying the expression or activity of a protein in a cell, the method comprising using at least one hardware processor for:
- analyzing multiple images of one or more labeled genetic entities;
- determining from said analysis, a motile property of said one or more labeled genetic entities;
- and
- identifying an expression or activity of a protein of said cell associated with said motile property.
A system for determining a cell characteristic, the system comprising:
- a microscope configured to magnify one or more labeled genetic entities;
- a camera configured to
- capture multiple images of said one or more labeled genetic entities, wherein said camera is coupled to said microscope;
- and
- a processor configured to
- analyze said multiple images;
- determine from said analysis, a motile property of said one or more labeled genetic entities;
- and
- identify an expression or activity of a nuclear protein of said cell associated with said motile property.
A method for determining the ratio of bound to unbound molecules in a cell, comprising:
- obtaining, from a selected area of a cell, data comprising time-resolved emitted light intensity measurements and time-resolved fluorescent lifetime measurements of fluorescently labeled bound molecules and fluorescently labeled unbound molecules;
- determining from the obtained data 1) a continuous photobleaching (CP) curve and 2) fluorescent lifetime histograms for each of the time-resolved emitted light intensity measurements and fluorescent lifetime measurements;
- and
- calculating a ratio of the fluorescently labeled bound molecules to the fluorescently labeled unbound molecules in the selected regions using the CP curve and the fluorescent lifetime histograms.
Dependencies are inferred from the claim text.