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US 2016/0238521 A1Published application
Simultaneous monitoring of fibrinogen and haematocrit in whole blood
AssigneesInstitut National de la Sante et de la Recherche Medical, Etat Francais (Ministere De La Defense), Service De Sante Des Armees, Assistance Publique-Hopitaux de Paris, Universite Paris Descartes
InventorsPascale GAUSSEM, Anne GODIER, Isabelle GOUIN-THIBAULT, Bernard LE BONNIEC, Anne-Celine MARTIN, Nyoucha MORRAVEDGE, Marc SAMAMA
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Abstract
The present invention relates to a method for determining both (a) the haematocrit value and (b) the fibrinogen content in whole blood of an individual in less than 5 minutes, as well as to associated portable apparatus.
Claims15
A method for determining both (a) the haematocrit value and (b) the fibrinogen content in whole blood of an individual comprising:
- (i) mixing a given volume of whole blood sample from the individual with a liquid solution, wherein the liquid solution comprises a low ionic strength buffer as well as a precipitant, and wherein the content of the precipitant ranges from 0.5 to 40% by weight based on the total weight of the solution, whereby a test mixture is obtained,
- the precipitant being selected from the group consisting of a (C1-C6)alcohol, acetaldehyde, acetic acid, acetone, acetonitrile, 2-butoxyethanol, butyric acid, diethanolamine, diethylenetriamine, dimethylformamide, dimethoxyethane, dimethyl sulfoxide, 1,4-dioxane, ethylamine, ethylene glycol, formic acid, furfuryl alcohol, methyl diethanolamine, methyl isocyanide, propanoic acid, pyridine and tetrahydrofuran;
- (ii) incubating the test mixture during 30 seconds or more;
- (iii) measuring light absorbance of the test mixture obtained at the end of step (ii) at a wavelength ranging from 520 to 590 nanometers whereby an absorbance value A is obtained;
- (iv) measuring light absorbance of the test mixture obtained at the end of step (ii) at a wavelength ranging from 650 to 850 nanometers, whereby an absorbance value B is obtained;
- (v) determining the haematocrit value of the individual by comparing the absorbance value A obtained at step (iii) with an haematocrit reference value;
- and
- (vi) determining the fibrinogen content in the whole blood of the individual by comparing the absorbance value B obtained at step (iv) with a fibrinogen content reference value;
- wherein the liquid solution used at step (i) and the test mixture used at steps (ii) to (iv) are maintained at a desired temperature, the desired temperature being selected in a temperature range of from 4 to 40° C.
6 more elements
A method for determining both (a) the haematocrit value and (b) the fibrinogen content in whole blood of an individual comprising the steps of:
- (i-a) mixing a given volume of a first whole blood sample from an individual with a first liquid solution, wherein the first liquid solution comprises a low ionic strength buffer as well as a precipitant, and wherein the content of the precipitant ranges from 0.5 to 20% by weight based on the total weight of the solution, whereby a first test mixture is obtained,
- the precipitant being selected from the group consisting of a (C1-C6)alcohol, acetaldehyde, acetic acid, acetone, acetonitrile, 2-butoxyethanol, butyric acid, diethanolamine, diethylenetriamine, dimethylformamide, dimethoxyethane, dimethyl sulfoxide, 1,4-dioxane, ethylamine, ethylene glycol, formic acid, furfuryl alcohol, methyl diethanolamine, methyl isocyanide, propanoic acid, pyridine and tetrahydrofuran;
- (ii-a) mixing another given volume of a second whole blood sample from the individual with a second liquid solution, wherein the second liquid solution comprises a low ionic strength buffer as well as a precipitant, and wherein the content of the precipitant ranges from 20 to 40% by weight based on the total weight of the solution, whereby a second test mixture is obtained,
- the precipitant being selected from the group consisting of a (C1-C6)alcohol, acetaldehyde, acetic acid, acetone, acetonitrile, 2-butoxyethanol, butyric acid, diethanolamine, diethylenetriamine, dimethylformamide, dimethoxyethane, dimethyl sulfoxide, 1,4-dioxane, ethylamine, ethylene glycol, formic acid, furfuryl alcohol, methyl diethanolamine, methyl isocyanide, propanoic acid, pyridine and tetrahydrofuran;
- (iii-a) incubating the first and second test mixtures during 30 seconds or more;
- (iv-a) measuring light absorbance of the first test mixture obtained at the end of step (iii-a) at a wavelength ranging from 520 to 590 nanometers, whereby an absorbance value A is obtained;
- (v-a) measuring light absorbance of the second test mixture obtained at the end of step (iii-a) at a wavelength ranging from 650 to 850 nanometers, whereby an absorbance value B is obtained;
- (vi-a) determining the haematocrit value of the individual by comparing the absorbance value A obtained at step (iv-a) with an haematocrit reference value;
- and
- (vii-a) determining the fibrinogen content in the whole blood of the individual by comparing the absorbance value B obtained at step (v-a) with fibrinogen content reference value;
- wherein the first and second liquid solutions used at steps (i-a) and (ii-a) and the first and second test mixtures used at steps (iii-a) to (v-a) are each independently maintained at a desired temperature, the desired temperature being selected in a temperature range of from 4 to 40° C.
8 more elements
Dependencies are inferred from the claim text.