Rny-derived small rnas as biomarkers for atherosclerosis-related disorders
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Universite Paul Sabatier Toulouse III, Universite De Nice Sophia Antipolis
InventorsMichele TRABUCCHI, Laurent MARTINEZ, Emanuela REPETTO
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Abstract
The present invention concerns an in vitro method of diagnosis or prognosis of an atherosclerosis-related disorder by detecting a small Y RNA (s-RNY), as well as the use of an inhibitor of s-RNY as a medicament against atherosclerosis-related disorders. The invention also concerns a method for screening for a compound suitable for the treatment of an atherosclerosis-related disorder.
Claims20
An in vitro method of diagnosis or prognosis of an atherosclerosis-related disorder in an individual, which comprises the steps of:
- a) determining the level of expression of at least one biomarker consisting of a small Y RNA (s-RNY) in a biological sample of said individual,
- b) comparing the level of expression of said at least one biomarker with a reference value,
- c) deducing from said comparison if the individual has developed or is at risk of developing an atherosclerosis-related disorder.
A method of screening for a compound suitable for the treatment of an atherosclerosis-related disorder, which comprises the steps of:
- a) contacting a candidate compound with a s-RNY or a RNY,
- b) identifying as a compound suitable for the treatment of an atherosclerosis-related disorder which inhibits the maturation of said RNY into s-RNY or which inhibits the activity of said s-RNY.
A method of treating an atherosclerosis-related disorder which comprises administering an inhibitor of a s-RNY to a subject in need thereof.
A method of treating an atherosclerosis-related disorder in an individual in need thereof, which comprises:
- a) diagnosing or prognosing the individual with an atherosclerosis-related disorder by measuring the level of expression of at least one biomarker consisting of a small Y RNA (s-RNY) in a biological sample of said individual;
- and
- b) administering a therapeutically effective amount of an anti-atherosclerosis drug to said individual diagnosed or prognosed with an atherosclerosis-related disorder.
An isolated nucleic acid consisting of sequence SEQ ID NO:
- 9 (s-RNY3-5p) or SEQ ID NO:
- 11 (s-RNY4-5p).
A pair of primers suitable for amplifying a nucleic acid sequence consisting of SEQ ID NO:
- 9 (s-RNY3-5p) or SEQ ID NO:
- 11 (s-RNY4-5p).
A probe for diagnosing or prognosing atherosclerosis-related disorder which is nucleic acid specifically hybridizing to a nucleic acid sequence consisting of SEQ ID NO:
- 9 (s-RNY3-5p) or SEQ ID NO:
- 11 (s-RNY4-5p).
Dependencies are inferred from the claim text.