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AssigneesPlant Response Biotech S.L., Consejo Superior de Investigaciones Cientificas
InventorsMarisé Borja, Julio Bonet-Gigante, Antonio Molina, Rafael Catalá, Julio Salinas
Estimating the term…
Methods of producing transgenic photosynthetic organisms and plants overexpressing an FMO protein are disclosed. The disclosure also relates to transgenic photosynthetic organisms and plants having between 4 and 37 fold greater expression of an FMO protein compared to wild-type plants, wherein said transgenic photosynthetic organisms and plants have between 1.1 and 3.4 fold greater trimethylamine N-oxide compared to wild-type, and wherein said transgenic photosynthetic organisms plants are drought tolerant. The disclosure further relates to DNA constructs and methods of producing DNA constructs having a promoter operably linked to one or more FMO protein coding sequences. The disclosure further relates to methods of producing drought tolerant plants and photosynthetic organisms by applying an effective amount of trimethylamine N-oxide di-hydrate.
A method of producing a transgenic photosynthetic organism or plant overexpressing an FMO protein, wherein the method comprises:
A DNA construct comprising:
A drought tolerant transgenic plant having one or more DNA constructs stably integrated into said plants genome, wherein said DNA construct comprises an FMO protein coding sequence operably linked to a promoter, wherein said plant overexpresses said FMO protein between 4 and 37 fold greater than the level of FMO expression in non-transgenic plants, wherein said overexpression of said FMO protein catalyzes the oxidation of endogenous metabolites containing nucleophilic nitrogen, and wherein said transgenic plant has between 1.1 and 3.4 fold greater trimethylamine N-oxide.
Dependencies are inferred from the claim text.