Soybean pip1 promoter and its use in constitutive expression of transgenic genes in plants
AssigneeE. I. Du Pont de Nemours and Company
InventorZhongsen Li
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Abstract
The disclosure relates to gene expression regulatory sequences from soybean, specifically to recombinant DNA constructs comprising the promoter of a soybean plasma membrane intrinsic protein gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
Claims20
A recombinant DNA construct comprising:
- (a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, or SEQ ID NO:6, or SEQ ID NO:
- 49, or a functional fragment thereof;
- or,
- (b) a full-length complement of (a);
- or,
- (c) a nucleotide sequence comprising a sequence having at least 71% sequence identity, based on the Clustal V method of alignment with pairwise alignment default parameters (KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4), when compared to the nucleotide sequence of (a);
- wherein said nucleotide sequence is a promoter.
A plant stably transformed with a recombinant DNA construct comprising a soybean constitutive promoter and a heterologous nucleic acid fragment operably linked to said constitutive promoter, wherein said constitutive promoter is a capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said constitutive promoter comprises any of the sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:49.
Dependencies are inferred from the claim text.