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US 2016/0237400 A1Published application
Isolation of non-embryonic stem cells and uses thereof
AssigneeThe Jackson Laboratory
InventorWa Xian
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AssigneeThe Jackson Laboratory
InventorWa Xian
Estimating the term…
The invention described herein relates to methods of isolating non-embryonic stem cell, e.g., adult stem cell, from a non-embryonic tissue, e.g., an adult tissue or organ. Non-embryonic stem cells (e.g., adult stem cells) thus isolated from the various tissues or organs can self-renew or propagate indefinitely in vitro, are multipotent and can differentiate into the various differentiated cell types normally found within the tissue or organ from which the stem cells are isolated. In addition, the isolated stem cells can be propagated through clonal expansion of a single isolated stem cell, to produce a clone of which at least about 40%, 70%, or 90% or more cells within the clone can be further passaged as single cell originated clones.
A method for isolating a non-embryonic stem cell from a non-embryonic tissue, the method comprising:
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A single cell clone of a non-embryonic stem cell, wherein at least about 40%, 50%, 60%, 70%, or about 80% of cells within said single cell clone, when isolated as single cell, is capable of proliferation to produce single cell clone.
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A single cell clone of a non-embryonic stem cell, wherein said non-embryonic stem cell expresses one or more stem cell markers selected from SOX9, KRT19, KRT7, LGR5, CA9, FXYD2, CDH6, CLDN18, TSPAN8, BPIFB1, OLFM4, CDH17, and PPARGC1A, such as a small intestine stem cell, which expresses one or more markers selected from:
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A medium for isolating and/or culturing non-embryonic stem cell, said medium comprising:
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Dependencies are inferred from the claim text.