2-deoxy-scyllo-inosose reductase

A protein of any of (a) through (e) below:

• (a) a protein comprising an amino acid sequence represented by SEQ ID NO:
• 2;
• (b) a protein comprising an amino acid sequence having 58% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 2 and having 2-deoxy-scyllo-inosose reductase activity;
• (c) a protein comprising an amino acid sequence having 56% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 4 and having 2-deoxy-scyllo-inosose reductase activity;
• (d) a protein comprising an amino acid sequence having 54% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 6 and having 2-deoxy-scyllo-inosose reductase activity;
• (e) a protein comprising an amino acid sequence having 54% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 8 and having 2-deoxy-scyllo-inosose reductase activity.

A gene comprising a nucleotide sequence of (a) or (b) below:

• (a) a nucleotide sequence represented by SEQ ID NO:
• 1;
• or
• (b) a nucleotide sequence hybridizing under stringent conditions with DNA comprising a sequence complementary to a nucleotide sequence comprising at least 18 consecutive bases in a nucleotide sequence represented by SEQ ID NO:
• 1 and encoding a protein having 2-deoxy-scyllo-inosose reductase activity.

A method for producing (−)-vibo-quercitol characterized in that a protein of any one of (a) through (e) below:

• (a) a protein comprising an amino acid sequence represented by SEQ ID NO:
• 2;
• (b) a protein comprising an amino acid sequence having 58% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 2 and having 2-deoxy-scyllo-inosose reductase activity;
• (c) a protein comprising an amino acid sequence having 56% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 4 and having 2-deoxy-scyllo-inosose reductase activity;
• (d) a protein comprising an amino acid sequence having 54% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 6 and having 2-deoxy-scyllo-inosose reductase activity;
• or
• (e) a protein comprising an amino acid sequence having 54% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 8 and having 2-deoxy-scyllo-inosose reductase activity is brought into contact with 2-deoxy-scyllo-inosose, reacted under conditions of pH 5.0-10.0, and the (−)-vibo-quercitol produced is recovered from the reaction solution.

A method for converting 2-deoxy-scyllo-inosose into (−)-vibo-quercitol characterized in that a protein of any one of (a) through (e) below:

• (a) a protein comprising an amino acid sequence represented by SEQ ID NO:
• 2;
• (b) a protein comprising an amino acid sequence having 58% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 2 and having 2-deoxy-scyllo-inosose reductase activity;
• (c) a protein comprising an amino acid sequence having 56% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 4 and having 2-deoxy-scyllo-inosose reductase activity;
• (d) a protein comprising an amino acid sequence having 54% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 6 and having 2-deoxy-scyllo-inosose reductase activity;
• or
• (e) a protein comprising an amino acid sequence having 54% or greater identity to an amino acid sequence represented by SEQ ID NO:
• 8 and having 2-deoxy-scyllo-inosose reductase activity is brought into contact with 2-deoxy-scyllo-inosose and reacted under conditions of pH 5.0-10.0.