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US 2016/0200761 A1Published application
Buoyant protein harvesting device
AssigneeTherapeutic Proteins International, LLC
InventorSarfaraz K. Niazi
Life of this patent
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Abstract
A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification.
Claims6
A method for harvesting refolded protein comprising:
- a. providing a protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein and a buoyancy device attached to the porous container;
- b. disposing the protein-harvesting device in a container comprising a protein in a refolding solution;
- c. adjusting the buoyancy of the protein-harvesting device to a pre-determined level;
- d. capturing a refolded protein from the refolding solution with the protein harvesting device;
- e. removing the refolding solution;
- f. eluting the refolded protein from the chromatography media of the protein harvesting device;
- and
- g. reconstituting the refolded protein eluted from the chromatography media for further purification.
5 more elements
A method for continuous harvesting proteins comprising:
- a. providing a protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein and a buoyancy device attached to the porous container;
- b. disposing the protein-harvesting device in a bioreactor comprising a genetically modified organism or cells capable of producing a protein for harvesting in a nutrient media at the beginning of protein production;
- c. adjusting the pH and electrolyte concentration of the nutrient media to optimize the binding of the protein to the chromatography media;
- d. capturing the protein from the nutrient media continuously throughout;
- and
- e. removing the protein-harvesting device from the nutrient media for further processing of the protein bound to the chromatography media.
3 more elements
A method for continuous harvesting proteins comprising:
- a. providing a protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein and a buoyancy device attached to the porous container;
- b. disposing the protein-harvesting device in a bioreactor comprising a genetically modified organism or cells capable of producing a protein for harvesting in a nutrient media at the beginning of protein production;
- c. adjusting the pH and electrolyte concentration of the nutrient media to optimize the binding of the protein to the chromatography media;
- d. capturing the protein from the nutrient media continuously throughout production of the target protein;
- and
- e. removing the protein-harvesting device from the nutrient media for further processing of the protein bound to the chromatography media.
3 more elements
A method comprising:
- a. continuously removing expressed protein from a nutrient media to enhance the level of expression that may be depressed because of the higher concentration of protein in the mixture;
- b. providing a protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein and a buoyancy device attached to the porous container;
- c. disposing the protein-harvesting device in a bioreactor comprising a genetically modified organism or cells capable of producing a protein for harvesting in a nutrient media at the beginning of protein production;
- d. adjusting the pH and electrolyte concentration of the nutrient media to optimize the binding of the protein to the chromatography media;
- e. capturing the protein from the nutrient media continuously throughout;
- and
- f. removing the protein-harvesting device from the nutrient media for further processing of the protein bound to the chromatography media.
4 more elements
A method comprising:
- a. continuously removing expressed protein from a nutrient media to reduce the toxicity of the expressed protein to host cells and thus prolonging the cycles of expression substantially increasing the yields of production;
- b. providing a protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein and a buoyancy device attached to the porous container;
- c. disposing the protein-harvesting device in a bioreactor comprising a genetically modified organism or cells capable of producing a protein for harvesting in a nutrient media at the beginning of protein production;
- d. adjusting the pH and electrolyte concentration of the nutrient media to optimize the binding of the protein to the chromatography media;
- e. capturing the protein from the nutrient media continuously throughout;
- and
- f. removing the protein-harvesting device from the nutrient media for further processing of the protein bound to the chromatography media.
4 more elements
A method comprising:
- a. increasing the chemical stability of expressed protein by binding it to a chromatography media as soon as it is expressed as the chemicals are always less stable in a solution form than in a solid form or in this case a complex form;
- b. providing a protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein and a buoyancy device attached to the porous container;
- c. disposing the protein-harvesting device in a bioreactor comprising a genetically modified organism or cells capable of producing a protein for harvesting in a nutrient media at the beginning of protein production;
- d. adjusting the pH and electrolyte concentration of the nutrient media to optimize the binding of the protein to the chromatography media;
- e. capturing the protein from the nutrient media continuously throughout;
- and
- f. removing the protein-harvesting device from the nutrient media for further processing of the protein bound to the chromatography media.
4 more elements
Dependencies are inferred from the claim text.