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US 2016/0068903 A1Published application
Selective Amplification of Nucleic Acid Sequences
AssigneeLC Sciences LC
InventorsXiaochuan Zhou, Qi Zhu, Nijing Sheng
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AssigneeLC Sciences LC
InventorsXiaochuan Zhou, Qi Zhu, Nijing Sheng
Estimating the term…
The present invention relates to the field of nucleic acid sequence replication including PCR. Specifically, the present invention relates to methods and compositions for amplifying one or more target sequences from one or more template sequences. In particular, the present invention provides novel primer designs to enhance specificity of PCR reactions. The present invention also provides methods and compositions to perform the selection of specific sequence sections using specific primers and the amplification of all selected sequence sections using a pair of common primers in a single reaction tube.
An oligonucleotide primer comprising a 3p arm having a 3′ end and a 5′ end, a loop section and a 5p arm having a 3′ end and a 5′ end, wherein the 5p arm hybridizes to a DNA template and wherein the 3p arm hybridizes to the DNA template and provides sequence specificity for polymerase extension and wherein the loop section is located between the 5p arm and the 3p arm and does not bind the DNA template.
A hybridization structure for assay use comprising a probe and a target wherein the hybridization structure has one or more single stranded loops and two or more duplex segments wherein each loop is located between the duplex segments.
20-60. (canceled)
Dependencies are inferred from the claim text.