Multiplexed bioassay techniques

A method comprising:

• providing a microfluidic device comprising a sensor and a substrate in which is formed a microchannel in fluid communication between an entry port and an exit port, wherein
• a first portion of the microchannel is configured to supply a plurality of different labeled probes, each selected to complex with one of a corresponding plurality of different analytes, and
• a second portion of the microchannel comprises a plurality of corresponding different supplementary probes covalently bound to the substrate, wherein a supplementary probe of the plurality of corresponding different supplementary probes is selected to bind to a part of a corresponding analyte of the corresponding plurality of different analytes, which part is exposed when the corresponding analyte is complexed with a corresponding labeled probe of the plurality of different labeled probes, and
• the sensor is configured to detect signals emitted. from the plurality of labeled probes in the second portion of the microchannel;
• moving a sample fluid from the entry port to the exit port;
• and
• obtaining data from the sensor that indicates measurements of the signals emitted from probes bound to the supplementary probes in the second portion during an observation period.

A kit comprising:

• a device comprising a sensor and a substrate in which is formed a microchannel in fluid communication between an entry port and an exit port, wherein
• a first portion of the microchannel is configured to supply a plurality of different labeled probes, each selected to complex with one of a corresponding plurality of different analytes, and
• a second portion of the microchannel configured for covalently binding a plurality of corresponding different supplementary probes to the substrate, wherein a supplementary probe of the plurality of corresponding different supplementary probes is selected to bind to a part of a corresponding analyte of the corresponding plurality of different analytes, which part is exposed when the corresponding analyte is complexed with a corresponding labeled probe of the plurality of different labeled probes;
• a supply of a plurality of molecules for each of the plurality of different labeled probes.