Microorganism having novel acrylic acid synthesis pathway and method of producing acrylic acid by using the microorganism
AssigneeSamsung Electronics Co., Ltd.
InventorsHunsu Chu, Jinho Ahn, Taewook Nam, Jiae Yun, Insuk Choi, Yeoju Song, Jinsuk Lee
Life of this patent
Estimating the term…
Abstract
A microorganism capable of producing an acrylic acid (AA), wherein activities of a pathway producing AA through conversions of 3-HP to 3-HP-CoA and 3-HP-CoA to AA-CoA in the microorganisms are increased; as well as a method of producing the microorganism and a method of producing an acrylic acid by using the same.
Claims20
A genetically engineered microorganism that produces acrylate, wherein the genetically engineered microorganism comprises:
- a genetic modification that increases CoA transferase activity in catalyzing conversion of 3-hydroxypropionic acid (3-HP) to 3-hydroxypropionyl-CoA (3-HP-CoA),
- a genetic modification that increases 3-HP-CoA dehydratase activity in catalyzing conversion of 3-HP-CoA to acrylyl-CoA, and
- a genetic modification that increases activity of an enzyme that catalyzes conversion of acrylyl-CoA to acrylate in the microorganism,
- compared to a microorganism of the same type that is not genetically engineered.
An expression vector encoding a CoA transferase, a 3-HP-CoA dehydratase, and an enzyme catalyzing conversion of acrylyl-CoA to acrylate.
Dependencies are inferred from the claim text.