Biocatalysts and methods for the synthesis of substituted lactams
AssigneeCodexis, Inc.
InventorsFabien Louis Cabirol, Haibin Chen, Anupam Gohel, Paulina Elena Salim, Derek J. Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Q. Pham
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
Claims9
An engineered polypeptide comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO:4 and one or more residue differences as compared to SEQ ID NO:4 selected from:
- X2M;
- X4F, X4L;
- X5C, X5F, X5M, X5P, X5Y;
- X6F, X6R, X6S, X6T;
- X8H;
- X9I, X9F, X9L;
- X10L;
- X12H;
- X13A, X13F;
- X17L;
- X18I;
- X21L;
- X22A, X22H;
- X25A;
- X27L, X27H, X27P;
- X28N;
- X29N, X29T;
- X30F, X30G;
- X31H, X31R;
- X34V;
- X37S;
- X42G;
- X43S;
- X46R;
- X47R;
- X48R, X48S, X48W;
- X49I, X49P;
- X50S;
- X52H;
- X54A, X54L, X54M, X54P, X54R;
- X55M;
- X56D, X56E, X56W;
- X61G;
- X61W;
- X62A, X62L, X62N, X62S;
- X64C;
- X66A, X66S;
- X68I;
- X69A, X69G, X69T;
- X72A;
- X80Q;
- X81K;
- X84T;
- X85L, X85R;
- X88W;
- X89L;
- X97P, X97T;
- X99L;
- X101G, X101L, X101R;
- X102H, X102R;
- X102T, X102W;
- X103G, X103N, X103V;
- X106L, X106S, X106T, X106V;
- X107V;
- X108T;
- X115R;
- X117V;
- X120F;
- X122F, X122M, X122W, X122Y;
- X124F, X124K, X124L, X124M, X124N, X124R, X124S, X124W;
- X126A, X126C, X126G, X126I, X126T;
- X127T;
- X128A;
- X131F;
- X132H;
- X134G, X134L, X134S, X134V, X134Y, X134W;
- X136F, X136K, X136L, X136M, X136Y;
- X139E;
- X140A, X140K;
- X140M, X140T, X140V;
- X141A;
- X142M;
- X143V;
- X144D, X144I;
- X150L, X150S;
- X152A, X152C, X152F, X152G, X152I, X152L, X152R, X152S, X152W;
- X155C, X155I, X155L, X155T;
- X156A, X156F, X156S, X156T;
- X157L;
- X159G;
- X160L;
- X161M, X161N;
- X165L, X165N, X165V;
- X168E, X168R;
- X176C;
- X179K;
- X185A;
- X190M;
- X191A, X191G, X191S;
- X192A, X192G, X192H, X192K, X192N, X192Q, X192R, X192S;
- X196L, X196V;
- X199L, X199V;
- X203M;
- X208K;
- X209F, X209M, X209Q, X209V;
- X210G;
- X215H, X215L;
- X217L;
- X223A, X223S, X223T, X223V;
- X227I;
- X231T;
- X233D;
- X234L;
- X241R;
- X256R;
- X260Q;
- X263V;
- X265Y, X265W;
- X266N;
- X267V;
- X269L, X269T;
- X270R, X270T;
- X273D, X273H, X273M, X273R, X273V;
- X274F, X274L, X274R, X274T;
- X282I, X282L, X282T, X282V;
- X284A, X284P, X284S, X284T, X284V;
- X295N, X295S;
- X296D, X296L, X296R, X296S, X296W;
- X300G, X300L;
- X305Q, X305T;
- X308A;
- X309L, X309T, X309W;
- X311Q, X311T;
- X312C, X312M;
- X316L, X316R;
- X319R, X319T, X319Y;
- X320G, X320Q;
- X323C, X323E, X323N, X323R;
- X324Q;
- X325M, X325P, X325Q, X325V;
- X327L, X327R;
- and X329P.
The engineered polypeptide of claim 11, in which the amino acid sequence further comprises at least one or more residue differences as compared to SEQ ID NO:4 selected from:
- X54A;
- X54L;
- X56D;
- X56E;
- X61G;
- X61W;
- X62A;
- X62L;
- X62N;
- X62S;
- X64C;
- X68I;
- X122F, X122W;
- X122Y;
- X124K;
- X124M;
- X124S;
- X124W;
- X126C;
- X126I;
- X136K;
- X136L;
- X136M;
- X139E;
- X140K;
- X143V;
- X150L;
- X155I;
- X155L;
- X159G;
- X160L;
- X176C;
- X192A;
- X192G;
- X192H;
- X192K;
- X192N;
- X192Q;
- X192R;
- X192S;
- X199L;
- X199V;
- X209F;
- X209M;
- X209Q;
- X209V;
- X223S;
- X223T;
- X223V;
- X227I;
- X282L, X282V;
- X284A;
- X284P;
- X284S;
- X284T;
- and X284V.
Dependencies are inferred from the claim text.