Engineered transcription activator like effector (tale) proteins

An isolated cell comprising a target site and a non-naturally occurring TALE DNA-binding protein that binds to the target site, the TALE DNA-binding protein comprising a plurality of TALE-repeat units, each TALE-repeat unit comprising a repeat variable di-residue region (RVD), and wherein

• (i) the RVD in the first repeat recognizes adenine (A);
• (ii) the target site does not comprise a 3′ adenine (A);
• (iii) the target site comprises 2 or fewer consecutive thymines (Ts);
• and
• (iv) the target site does not contain 5′-GG-3′ or 5′-GC-3′ dinucleotides.