Method to Develop Herbicide-Resistant Sugar Beet Plants
AssigneeSESVanderHave NV
InventorsGuy WEYENS, Marc LEFÈBVRE, Rüdiger HAIN, Gerhard JOHANN
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Abstract
A method for producing herbicide-resistant sugar beet plant includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. A composition having an ALS herbicide is applied to the cells at a concentration which is lethal to the cells. Sugar beet plants are regenerated from the surviving cells.
Claims24
A method for producing a mutant sugar beet plant being resistant to one or more inhibitor(s) of the acetohydroxyacid synthase enzyme (ALS) comprising the steps of:
- obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant;
- applying to an in vitro culture of said protoplasts a composition comprising one or more ALS inhibitor(s) at a concentration that is lethal to more than 99% of the in vitro cultured cells;
- and
- regenerating sugar beet plants from the surviving cells of said in vitro cultured cells,
- wherein said stomatal guard cells protoplasts are pre selected for their capacity to regenerate into a sugar beet plant, and wherein said ALS inhibitor(s) is/are applied to more than 20 000 000 of said protoplasts.
A method for producing a mutant sugar beet plant being resistant to an herbicide comprising the steps of:
- obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant;
- applying to an in vitro culture of said protoplasts a composition comprising said herbicide at a concentration that is lethal to more than 99% of the in vitro cultured cells;
- regenerating sugar beet plants from the surviving cells of said in vitro cultured cells;
- and
- selecting regenerated sugar beet plants having a mutation in the gene encoding the peptide targeted by said herbicide,
- wherein said stomatal guard cells protoplasts are pre selected for their capacity to regenerate into a sugar beet plant, and wherein said herbicide is applied to more than 20 000 000 of said protoplasts.
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A mutated sugar beet plant comprising SEQ.ID.NO:3 on one allele of the ALS gene and SEQ.ID.NO:5 on the second allele of the ALS gene.
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Dependencies are inferred from the claim text.