Aptamer method
AssigneeOxford Nanopore Technologies PLC
InventorsDaniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
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Abstract
The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
Claims43
A method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes, the method comprising:
- (a) contacting the sample with a transmembrane pore and a panel of two or more probes,
- wherein each probe recognises one or more of the analyte members and comprises (i) an aptamer that binds to one or more of the analyte members and (ii) a tail which is capable of entering the pore and has different effects on the current flowing through the pore depending on whether or not the aptamer in the probe is bound to one of the analyte members,
- wherein each probe affects the current flowing through the pore in a distinctive manner, and
- wherein each analyte member of the group of two or more analytes is recognised by at least one probe in the panel;
- and
- (b) measuring the current flowing through the pore to determine which probes in the panel, if any, have bound to an analyte member and thereby determining the presence or absence of one or more analyte members in the sample.
A panel of probes for determining in a sample the presence, concentration or absence of one or more analyte members of a group of two or more analytes, the panel comprising two or more probes,
- wherein each probe recognises one or more of the analyte members and comprises (i) an aptamer that binds to one or more of the analyte members and (ii) a tail which is capable of entering a transmembrane pore and has different effects on the current flowing through the pore depending on whether or not the aptamer in the probe is bound to one of the analyte members,
- wherein each probe affects the current flowing through the pore in a distinctive manner, and
- wherein each analyte member in the group of two or more analytes is recognised by at least one probe in the panel.
Dependencies are inferred from the claim text.