Method For Coding Of Multiple PCR Reactions For Assay Recognition
AssigneeRoche Molecular Systems, Inc.
InventorsAmar Gupta, Nancy Schoenbrunner, Kevin Janssen, Nicolas Newton
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Abstract
The present invention provides for methods and compositions that use fluorescent dyes for the identification of reagents and solutions that are used to perform PCR assays.
Claims10
A method for preparing a plurality of reaction mixtures for performing polymerase chain reaction (PCR) amplification of a plurality of target nucleic acids, comprising:
- providing a first mastermix solution comprising at least one substance required for performing PCR amplification of a first target nucleic acid, and further comprising a first fluorescent dye and a second fluorescent dye, wherein said first dye and said second dye are present at a predetermined concentration ratio that is unique for said first reagent solution;
- providing a second mastermix solution comprising at least one substance required for performing PCR amplification of a second target nucleic acid, and further comprising said first fluorescent dye and said second fluorescent dye, wherein said first dye and said second dye are present at a predetermined concentration ratio different from the concentration ratio for said first mastermix solution and that is unique for said second mastermix solution;
- adding said first mastermix solution to a first reaction mixture to perform PCR amplification of said first target nucleic acid;
- and
- adding said second mastermix solution to a second reaction mixture to perform PCR amplification of said second target nucleic acid.
A method for encoding the identity of a mastermix solution used for performing polymerase chain reaction (PCR) amplification of a target nucleic acid, comprising:
- mixing together in the mastermix solution a first fluorescent dye and a second fluorescent dye present at a predetermined concentration ratio that produces a fluorescence ratio of the first fluorescent dye over the second fluorescent dye that is distinguishable from fluorescence ratios produced from other mastermix solutions;
- detecting the fluorescence from said first fluorescent dye and from said second fluorescent dye;
- and
- determining said fluorescence ratio, thereby identifying said mastermix solution.
A kit for performing polymerase chain reaction (PCR) amplification of a plurality of target nucleic acids, comprising a plurality of mastermix solutions wherein each one mastermix solution from the plurality of mastermix solutions comprises at least one substance required for performing PCR amplification of a specific target nucleic acid, and further comprises a first fluorescence dye and a second fluorescence dye;
- and wherein for said each one mastermix solution, said first fluorescence dye and said second fluorescence dye are present at a concentration ratio that is different and produces a fluorescence ratio that is distinguishable from every other mastermix solution from the plurality of mastermix solutions.
Dependencies are inferred from the claim text.