Method for Identifying Bacteria from the Bacillus Cereus Group

Abstract

The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in: bringing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C substrate and an inhibitor of Gram-negative bacteria into contact, in a container; incubating all of the above together; identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction carried out are adapted in such a way that said hydrolysis reaction by the bacteria of the Bacillus cereus group is detected before the hydrolysis of the PC-PLC substrate by the Gram-positive bacteria other than those belonging to the Bacillus cereus group, and potentially present in the sample, is detectable.

Dependencies are inferred from the claim text.