Reactive oxygen species-resistant microorganisms
AssigneeJoule Unlimited Technologies, Inc.
InventorsMichael Connor, Frank A. Skraly
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Abstract
The present disclosure identifies pathways and mechanisms to confer resistance to reactive oxygen species, e.g., hydrogen peroxide, to photoautotrophic organisms. The use of such organisms in host cell culture systems with high levels of reactive oxygen species, such as host cell culture systems decontaminated with hydrogen peroxide, is contemplated.
Claims23
A method to produce a carbon-based product of interest, comprising culturing an engineered cyanobacterial cell in a cell culture in the presence of CO2 and light under conditions suitable to produce a carbon-based product of interest, wherein said engineered cyanobacterial cell comprises a recombinant nucleic acid encoding an enzyme classified under an Enzyme Commission number selected from EC 1.11.1.6, EC 1.11.1.7, and EC 1.11.1.21.
13-15. (canceled)
17-18. (canceled)
A method for conferring hydrogen peroxide resistance to a parent cyanobacterial cell, comprising:
- a. transforming said parent cyanobacterial cell with a nucleic acid encoding an enzyme selected from KatG and KatE, resulting in an engineered cyanobacterial cell;
- and
- b. culturing said engineered cyanobacterial cell in a medium comprising hydrogen peroxide.
20-26. (canceled)
An engineered host cell comprising a recombinant gene encoding a first enzyme classified under an Enzyme Commission number selected from EC 1.11.1.6, EC 1.11.1.7, and EC 1.11.1.21, wherein said host cell further comprises a recombinant pyruvate decarboxylase or a recombinant alcohol dehydrogenase.
Dependencies are inferred from the claim text.