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AssigneeCytonics Corporation
InventorsLewis Hanna, John David Laughlin, Shawn Robert Browning
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Systems and methods for purification and concentration of autologous alpha-2-macroglobulin (A2M) from whole blood are provided. Also provided are diagnostic methods for identifying sites in the synovial joints, spine, tendons or ligaments for treatment of pain, degeneration, or inflammation with autologous A2M. Methods for utilizing autologous A2M in combination with other autologous treatments (e.g. platelets and other growth factors) are provided in addition to combinations with exogenous drugs or carriers. Also provided is a method of producing recombinant A2M wild type or variants thereof where the bait region was modified to enhance the inhibition characteristics of A2M and/or to prolong the half life of the protein in joints and spine disc or epidural space.
1-202. (canceled)
A liquid composition comprising:
A method comprising:
A method of treating an injury or a disease or condition whose pathogenesis comprises protease activity comprising administering to a mammal in need thereof a composition, wherein the composition comprises
A composition comprising a recombinant A2M comprising a non-natural bait that replaces a wild-type A2M bait region, wherein the recombinant A2M is characterized by at least a 10% enhanced inhibition of a protease compared an inhibition of the protease by an equivalent amount of a wild-type A2M, wherein the non-natural bait region comprises a recognition site for a protease that is not present in a wild-type A2M.
Dependencies are inferred from the claim text.