Systems and methods for detection of cells using engineered transduction particles

A method, comprising:

• coupling a reagent module to a sample container such that a reaction chamber defined by the sample container is fluidically isolated from an exterior volume, the reaction chamber containing a sample, the reagent module including a reagent container containing a reagent;
• disposing, after the coupling, at least a distal end portion of the sample container into a detection volume of an instrument;
• puncturing, when the distal end portion of the sample container is in the detection volume, the reagent container to convey the reagent from the reagent module into the reaction chamber of the sample container, the reagent formulated to contact the sample to enhance production of a luminescence reaction;
• and
• receiving, during the conveying, a signal associated with the luminescence reaction.

A method, comprising:

• disposing a distal end portion of a sample container into a detection volume of an instrument, the sample container being coupled to a reagent module such that a reaction chamber defined by the sample container is fluidically isolated from an exterior volume, the reaction chamber containing a sample;
• applying a first force on the reagent module such that movement of the sample container in at least one direction is limited;
• applying a second force on the reagent module to convey a reagent from the reagent module into the reaction chamber of the sample container;
• and
• receiving, during at least one of the applying the first force or the applying the second force, a signal associated with a magnitude of light emission from the reaction chamber.