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US 2015/0147809 A1Published application
Cell seeding device and method
AssigneeVericel Corporation
InventorsYongzhong WANG, Christopher KELLY, Stephen RAPKO, Frank QUINNO, Barbara SEYMOUR
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Abstract
Described is a device comprising an anchor, a tray including a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well. The device may be used to form a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions.
Claims20
A device comprising:
- an anchor;
- a tray including a well adapted to receive the anchor;
- and
- a cover adapted to engage the tray and cover the well.
1 more element
A device comprising:
- an anchor including at least one first sidewall enclosing a space having X, Y, and Z dimensions;
- a tray including a well adapted to receive the anchor, the well having at least one second sidewall and a base having X and Y dimensions;
- and
- a cover adapted to engage the tray,
- wherein the X and Y dimensions of the space are approximately equal to the X and Y dimensions of the base such that when the anchor is in the well, at least one of the at least one first sidewall and at least one of the at least one second sidewall are in direct contact.
2 more elements
A method of generating a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions comprising:
- providing a device comprising:
- an anchor;
- and
- a tray including a well adapted to receive the anchor;
- placing a membrane in the well;
- placing the anchor in the well at least partially in contact with the membrane;
- and
- filling the well at least partially with a medium containing cells.
5 more elements
The method according to claim 20, further comprising:
- removing the anchor from the well;
- and
- removing the membrane-cell matrix.
Dependencies are inferred from the claim text.