Ethane-1,2-diol producing microorganism and a method for producing ethane-1,2-diol from d-xylose using the same
AssigneeMyongji University Industry and Academia Cooperation Foundation
InventorsWook-Jin Chung, Huaiwei Liu
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Abstract
Disclosed herein is a microorganism capable of producing ethane-1,2-diol from D-xylose, and a method for producing ethane-1,2-diol using the same. More specifically, the present invention relates to an engineered Escherichia coli (E. coli) prepared by knocking out a D-xylose isomerase gene and/or an aldehyde dehydrogenase gene within the genomic DNA of E. coli and transforming an expression vector including a D-xylose dehydrogenase gene into the E. coli, and an efficient method for producing ethane-1,2-diol from D-xylose using the engineered E. coli.
Claims19
An engineered Escherichia coli (E. coli) capable of producing ethane-1,2-diol from D-xylose by knocking out D-xylose isomerase gene, xylA, within the genomic DNA of E. coli followed by transforming an expression vector including D-xylose dehydrogenase gene, cxylB, into the xylA-knockout E. coli.
A method of preparing an engineered E. coli capable of producing ethane-1,2-diol from D-xylose, comprising:
- 1) knocking out D-xylose isomerase gene, xylA, from a given E. coli;
- 2) constructing an expression vector including xylose dehydrogenase gene, cxylB;
- and
- 3) transforming the resulting expression vector in step 2) into the E. coli in step 1).
Dependencies are inferred from the claim text.