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US 2015/0147790 A1Published application
Acyl-acp thioesterase genes and uses therefor
AssigneeSynthetic Genomics, Inc.
InventorsPaul Gordon Roessler, Gena Roy
Life of this patent
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Abstract
The present invention provides novel genes encoding Class II acyl-ACP thioesterases and variants thereof that are active on C8, C10, C12, C14, C16, and C18 acyl-ACP substrates. The thioesterases can be introduced into transgenic organisms, including microorganisms and photosynthetic organisms, for producing fatty acids and fatty acid products.
Claims25
A transgenic organism comprising an exogenous nucleic acid molecule encoding a Class II acyl-ACP thioesterase comprising an amino acid sequence selected from the group consisting of:
- a) an amino acid sequence having at least 85% identity to amino acid 64 to amino acid 361 of SEQ ID NO:51;
- b) an amino acid sequence having at least 98% identity to amino acid 66 to amino acid 362 of SEQ ID NO:55;
- c) an amino acid sequence having at least 97% identity to amino acid 65 to amino acid 360 of SEQ ID NO:59;
- d) an amino acid sequence having at least 90% identity to amino acid 65 to amino acid 359 of SEQ ID NO:63;
- e) an amino acid sequence having at least 98% identity to amino acid 115 to amino acid 410 of SEQ ID NO:65;
- f) an amino acid sequence having at least 96% identity to amino acid 65 to amino acid 356 of SEQ ID NO:69;
- g) an amino acid sequence having at least 98% identity to amino acid 115 to amino acid 410 of SEQ ID NO:71;
- h) an amino acid sequence having at least 96% identity to amino acid 64 to amino acid 361 of SEQ ID NO:75;
- i) an amino acid sequence having at least 97% identity to amino acid 65 to amino acid 360 of SEQ ID NO:79;
- j) an amino acid sequence having at least 96% identity to amino acid 116 to amino acid 413 of SEQ ID NO:81;
- k) an amino acid sequence having at least 96% identity to amino acid 65 to amino acid 362 of SEQ ID NO:85;
- l) an amino acid sequence having at least 96% identity to amino acid 64 to amino acid 361 of SEQ ID NO:89;
- m) an amino acid sequence having at least 97% identity to amino acid 115 to amino acid 394 of SEQ ID NO:91;
- n) an amino acid sequence having at least 97% identity to amino acid 115 to amino acid 394 of SEQ ID NO:93;
- o) an amino acid sequence having at least 99% identity to amino acid 115 to amino acid 394 of SEQ ID NO:95;
- p) an amino acid sequence having at least 92% identity to amino acid 63 to amino acid 360 of SEQ ID NO:99;
- and
- q) an amino acid sequence having at least 98% identity to amino acid 115 to amino acid 393 of SEQ ID NO:101.
15 more elements
A vector comprising a recombinant nucleic acid molecule encoding a Class II acyl-ACP thioesterase comprising an amino acid sequence selected from the group consisting of:
- a) an amino acid sequence having at least 99% identity to amino acid 65 to amino acid 355 of SEQ ID NO:29;
- b) an amino acid sequence having at least 85% identity to amino acid 64 to amino acid 361 of SEQ ID NO:51;
- c) an amino acid sequence having at least 98% identity to amino acid 66 to amino acid 362 of SEQ ID NO:55;
- d) an amino acid sequence having at least 97% identity to amino acid 65 to amino acid 360 of SEQ ID NO:59;
- e) an amino acid sequence having at least 90% identity to amino acid 65 to amino acid 359 of SEQ ID NO:63;
- f) an amino acid sequence having at least 98% identity to amino acid 115 to amino acid 410 of SEQ ID NO:65;
- g) an amino acid sequence having at least 96% identity to amino acid 65 to amino acid 356 of SEQ ID NO:69;
- h) an amino acid sequence having at least 98% identity to amino acid 115 to amino acid 410 of SEQ ID NO:71;
- i) an amino acid sequence having at least 96% identity to amino acid 64 to amino acid 361 of SEQ ID NO:75;
- j) an amino acid sequence having at least 97% identity to amino acid 65 to amino acid 360 of SEQ ID NO:79;
- k) an amino acid sequence having at least 96% identity to amino acid 116 to amino acid 413 of SEQ ID NO:81;
- l) an amino acid sequence having at least 96% identity to amino acid 65 to amino acid 362 of SEQ ID NO:85;
- m) an amino acid sequence having at least 96% identity to amino acid 64 to amino acid 361 of SEQ ID NO:89;
- n) an amino acid sequence having at least 97% identity to amino acid 115 to amino acid 394 of SEQ ID NO:91;
- o) an amino acid sequence having at least 97% identity to amino acid 115 to amino acid 394 of SEQ ID NO:93;
- p) an amino acid sequence having at least 99% identity to amino acid 115 to amino acid 394 of SEQ ID NO:95;
- q) an amino acid sequence having at least 92% identity to amino acid 63 to amino acid 360 of SEQ ID NO:99;
- and
- r) an amino acid sequence having at least 98% identity to amino acid 115 to amino acid 393 of SEQ ID NO:101.
- wherein the vector further comprises a selectable marker.
17 more elements
Dependencies are inferred from the claim text.