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US 2015/0147753 A1Published application
Method for producing hyperactive transposase mutants
AssigneeHarri SAVILAHTI
InventorsHarri Savilahti, Tiina Rasila
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AssigneeHarri SAVILAHTI
InventorsHarri Savilahti, Tiina Rasila
Estimating the term…
The present invention provides a method for producing a hyperactive MuA transposase variant comprising at least one single-amino-acid change, the method comprising the steps of modifying the nucleic acid encoding wild type MuA transposase in at least one of the positions 59, 97, 160, 179, 233, 254, 258, 302, 335, 340, 345, 374, 447, 464, 478, 482, 483, 487, 495, 507, 539, 594 or 617 so that the modified nucleic acid encodes a MuA transposase variant comprising at least one single-amino-acid change in its amino acid sequence, wherein said single-amino-acid change results in higher enzyme activity of the variant when compared to the wild type MuA transposase. The present invention also provides hyperactive MuA transposases and kits comprising the same.
-19. (canceled)
A method for producing a hyperactive MuA transposase variant comprising at least one single-amino-acid-change, the method comprising the steps of modifying the nucleic acid encoding wild type MuA transposase so that the modified nucleic acid encodes a MuA transposase variant comprising at least one single-amino-acid change at the amino acid positions of SEQ ID NO:1, producing a MuA transposase variant by expressing the modified nucleic acid in a host cell and identifying those MuA variants the transposase activity of which is higher than the transposase activity of the wild type MuA.
A hyperactive MuA transposase comprising at least one single-amino-acid change at the amino acid positions of SEQ ID NO:1 selected from the group consisting of:
Dependencies are inferred from the claim text.